Evidence mapPaperPMID 23201900Full record

ArticleNature chemical biology2013

Whole-organism screening for gluconeogenesis identifies activators of fasting metabolism.

Philipp Gut, Bernat Baeza-Raja, Olov Andersson, Laura Hasenkamp, Joseph Hsiao, Daniel Hesselson, Katerina Akassoglou, Eric Verdin, Matthew D Hirschey, Didier Y R Stainier

Open access · greenAbstract read
In one paragraph

Article in Nature chemical biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 94 papers.

0numbers the graph read from it
0cells of the map it votes in
94citing papers in PubMed
7.3field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

94 citing papers in PubMed, 185 citations in OpenAlex.

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34 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 2 countries.

Philipp GutDepartment of Biochemistry and Biophysics, University of California-San Francisco, San Francisco, California, USA. philipp.gut@ucsf.edu
Bernat Baeza-Raja
Olov Andersson
Laura Hasenkamp
Joseph Hsiao
Daniel Hesselson
Katerina Akassoglou
Eric Verdin
Matthew D Hirschey
Didier Y R Stainier
California Institute for Regenerative Medicine · USGladstone Institutes · USUniversity of California, San Francisco · USDuke University · US

Funding

UCSF-GIVI Center for AIDS researchP30AI027763 · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · 1988 to 2025
$12.8M
UCSF Liver CenterP30DK026743 · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · 1986 to 2025
$6.5M
Vanderbilt Mouse Metabolic Physiology CenterU24DK059637 · VANDERBILT UNIVERSITY · 2001 to 2005
$5.3M
PILOT &FEASIBILTY 2P30DK063720 · UNIVERSITY OF CALIFORNIA SAN FRANCISCO · 2003 to 2005
$3.6M
DIABETES, ENDOCRINOLOGY &METABOLISM TRAINING PROGRAMT32DK007418 · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · 1986 to 2025
$3.5M
MOLECULAR GENETICS OF LIVER DEVELOPMENTR01DK060322 · UNIVERSITY OF CALIFORNIA SAN FRANCISCO · 2001 to 2005
$1.7M
INTERACTIONS OF FIBRINOGEN WITH p75NTRR01NS051470 · UNIVERSITY OF CALIFORNIA SAN DIEGO · 2005 to 2005
$350k
NIAID NIH HHS P30 AI027763NIDDK NIH HHS DK59637NIDDK NIH HHS P30 DK026743NIDDK NIH HHS P30 DK063720NIDDK NIH HHS R01 DK060322NIDDK NIH HHS R01 DK60322NIDDK NIH HHS T32 DK007418NIDDK NIH HHS U01 DK089541NIDDK NIH HHS U24 DK059637NINDS NIH HHS NS051470NINDS NIH HHS R01 NS051470
6 · The paper itself

Abstract

Improving the control of energy homeostasis can lower cardiovascular risk in metabolically compromised individuals. To identify new regulators of whole-body energy control, we conducted a high-throughput screen in transgenic reporter zebrafish for small molecules that modulate the expression of the fasting-inducible gluconeogenic gene pck1. We show that this in vivo strategy identified several drugs that affect gluconeogenesis in humans as well as metabolically uncharacterized compounds. Most notably, we find that the translocator protein ligands PK 11195 and Ro5-4864 are glucose-lowering agents despite a strong inductive effect on pck1 expression. We show that these drugs are activators of a fasting-like energy state and, notably, that they protect high-fat diet-induced obese mice from hepatosteatosis and glucose intolerance, two pathological manifestations of metabolic dysregulation. Thus, using a whole-organism screening strategy, this study has identified new small-molecule activators of fasting metabolism.

Indexed as

Food DeprivationAnimalsAnimals, Genetically ModifiedBenzodiazepinonesDrug DesignFastingGluconeogenesisGlucoseHumansIntracellular Signaling Peptides and ProteinsIsoquinolinesLigandsMaleMiceMice, Inbred C57BLMice, Obese4'-chlorodiazepamBenzodiazepinonesGlucoseIntracellular Signaling Peptides and ProteinsIsoquinolinesLigandsPCK1 protein, humanPhosphoenolpyruvate Carboxykinase (GTP)PK 11195

Identifiers

PMID23201900
PMCPMC3552031
OpenAlexW2027976634

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.