ArticleJournal of proteome research2013
Proteomic analysis of Trypanosoma cruzi secretome: characterization of two populations of extracellular vesicles and soluble proteins.
Article in Journal of proteome research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 163 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
163 citing papers in PubMed, 256 citations in OpenAlex.
- Extracellular Vesicles ofJournal of extracellular biology · 2026Article
- Transcriptomic response to different heme sources inMicrobial cell (Graz, Austria) · 2026Article
- Cardiac alterations induced by Trypanosoma cruzi extracellular vesicles and immune complexes.PLoS neglected tropical diseases · 2025Article
- Mass spectrometry-based discovery and diagnostic validation of T. cruzi antigens in the urine of congenitally infected Chagas Disease patients.PLoS neglected tropical diseases · 2025Article
- An Overview ofPathogens (Basel, Switzerland) · 2025Review
- An algorithm for annotation and classification of T. cruzi MASP sequences: towards a better understanding of the parasite genetic variability.BMC genomics · 2025Article
- Trypanosomatid Extracellular Vesicles as Potential Immunogens for Chagas Disease.International journal of molecular sciences · 2025Article
- Review
- Towards a map of the immune system manipulation network byFrontiers in cellular and infection microbiology · 2025Review
- NinoaPathogens (Basel, Switzerland) · 2024Article
- Circulating extracellular vesicles in sera of chronic patients as a method for determining active parasitism in Chagas disease.PLoS neglected tropical diseases · 2024Article
- Analysis of virulence factors in extracellular vesicles secreted by Naegleria fowleri.Parasitology research · 2024Article
- Inorganic Polyphosphate Is in the Surface ofPathogens (Basel, Switzerland) · 2024Article
- Molecular similarities between the genes for Trypanosoma cruzi microtubule-associated proteins, mammalian interferons, and TRIMs.Parasitology research · 2024Article
- Potential of extracellular vesicles in the pathogenesis, diagnosis and therapy for parasitic diseases.Journal of extracellular vesicles · 2024Review
- Insights into Peptidyl-ProlylPathogens (Basel, Switzerland) · 2024Review
- Progress on the Regulation of the Host Immune Response by Parasite-Derived Exosomes.Pathogens (Basel, Switzerland) · 2024Review
- The End Justifies the Means: Chagas Disease from a Perspective of the Host-Life (Basel, Switzerland) · 2024Review
- Oxidative stress-induced changes in the transcriptomic profile of extracellular vesicles.Journal of extracellular biology · 2024Article
- Trypanosoma cruzi heme responsive gene (TcHRG) plays a central role in orchestrating heme uptake in epimastigotes.The FEBS journal · 2024Article
103 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
Abstract
Microorganisms use specialized systems to export virulence factors into host cells. Secretion of effector proteins into the extracellular environment has been described in Trypanosoma cruzi; however, a comprehensive proteomic analysis of the secretome and the secretion mechanisms involved remain elusive. Here, we present evidence that T. cruzi releases proteins associated with vesicles that are formed by at least two different mechanisms. Transmission electron microscopy showed larger vesicles budding from the plasma membrane of noninfective epimastigotes and infective metacyclic trypomastigotes, as well as smaller vesicles within the flagellar pocket of both forms. Parasite conditioned culture supernatant was fractionated and characterized by morphological, immunochemical, and proteomic analyses. Three fractions were obtained by differential ultracentrifugation: the first enriched in larger vesicles resembling ectosomes, the second enriched in smaller vesicles resembling exosomes, and a third fraction enriched in soluble proteins not associated with extracellular vesicles. Label-free quantitative proteomic analysis revealed a rich collection of proteins involved in metabolism, signaling, nucleic acid binding, and parasite survival and virulence. These findings support the notion that T. cruzi uses different secretion pathways to excrete/secrete proteins. Moreover, our results suggest that metacyclic forms may use extracellular vesicles to deliver cargo into host cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.