ArticleCell transplantation2013
Systematic analysis of donor and isolation factor's impact on human islet yield and size distribution.
Article in Cell transplantation, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- Intraparenchymal Enzyme Injections in Islet Isolations With Incomplete Ductal Perfusion of Enzymes.Transplant international : official journal of the European Society for Organ Transplantation · 2025Article
- Characterizing the effects of Dechlorane Plus on β-cells: a comparative study across models and species.Islets · 2024Article
- High-Efficiency, Prevascularization-Free Macroencapsulation System for Subcutaneous Transplantation of Pancreatic Islets for Enhanced Diabetes Treatment.Advanced materials (Deerfield Beach, Fla.) · 2024Article
- Review
- Evaluating Global and Temporal Trends in Pancreas and Islet Cell Transplantation: Public Awareness and Engagement.Clinics and practice · 2024Article
- Donor- and isolation-related predictive factors ofFrontiers in endocrinology · 2024Article
- Role of Mesenchymal Stem/Stromal Cells in Modulating Ischemia/Reperfusion Injury: Current State of the Art and Future Perspectives.Biomedicines · 2023Review
- A predictive computational platform for optimizing the design of bioartificial pancreas devices.Nature communications · 2022Article
- Article
- Clinical use of donation after circulatory death pancreas for islet transplantation.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2021Article
- Saturated fatty acids entrap PDX1 in stress granules and impede islet beta cell function.Diabetologia · 2021Article
- Development and validation of a quantitative electron microscopy score to assess acute cellular stress in the human exocrine pancreas.The journal of pathology. Clinical research · 2021Article
- A Smartphone-Fluidic Digital Imaging Analysis System for Pancreatic Islet Mass Quantification.Frontiers in bioengineering and biotechnology · 2021Article
- Human Pluripotent Stem Cells: A Unique Tool for Toxicity Testing in Pancreatic Progenitor and Endocrine Cells.Frontiers in endocrinology · 2020Article
- Validation of a New North American Islet Donor Score for Donor Pancreas Selection and Successful Islet Isolation in a Medium-Volume Islet Transplant Center.Cell transplantation · 2019Article
- The Flaws and Future of Islet Volume Measurements.Cell transplantation · 2018Review
- Influence of organ donor attributes and preparation characteristics on the dynamics of insulin secretion in isolated human islets.Physiological reports · 2018Article
- Improved yield of canine islet isolation from deceased donors.BMC veterinary research · 2017Article
- Human islet preparations distributed for research exhibit a variety of insulin-secretory profiles.American journal of physiology. Endocrinology and metabolism · 2015Article
- Islet product characteristics and factors related to successful human islet transplantation from the Collaborative Islet Transplant Registry (CITR) 1999-2010.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2014Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Islet transplantation is a promising therapy for T1DM. Key factors influencing islet yield have been identified with conflicting results. In this study, we analyzed 276 isolations to identify variables for islet yield and, additionally, islet size and size distribution. Pearson correlation analyses demonstrated that BMI had a positive correlation with pancreas size, actual islet count (AIC), and islet equivalent (IEQ)/g (all p ≤ 0.009), while CIT had a negative correlation with AIC and IEQ/g (all p ≤ 0.003). In mixed linear regression, BMI also had a positive correlation with islet size but only for shorter digestion times (≤15 min); there was no association between BMI and islet size for longer digestion times (>15 min). CIT was not associated with islet size. Donor age, sex, and preservation solutions were shown to have no correlation with islet yields or size distribution. Pancreas size had a positive correlation with AIC and a negative association with IEQ/g; it also had positive association with islet size but only for females, not males. Overdigestion was positively associated with islet counts; however, there was also a greater proportion of smaller islets when digestion rate was >74% (p = 0.005). Of the three collagenases analyzed, Sigma V had the lowest digestion rate (mean = 65%), approximately 5% or 10% lower than Roche Liberase HI (p = 0.04) and Serva NB1 (p = 0.0003), respectively; however, the Sigma V group showed better islet size preservation. Yet, the enzymes resulted in similar IEQ/g digested tissue. Of the isolated islets, 70.2% were smaller than 150 µm and contributed only 20.4% to the total IEQ, while 7.4% of the islets were larger than 250 µm but contributed 42.4% to the total IEQ. In summary, BMI, pancreas size, and CIT are useful variables for predicting islet yield, but selection of enzyme and balancing digestion time and rate are also important.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.