ArticleBMC genomics2013
The genome and transcriptome of perennial ryegrass mitochondria.
Article in BMC genomics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 40 citations in OpenAlex.
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- Phylogenetic reconstruction and species delimitation in Stipeae with special reference to Stipa (Poaceae, Pooideae) using mitochondrial genomes.Cladistics : the international journal of the Willi Hennig Society · 2025Article
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- Complete mitochondrial genome of Agrostis stolonifera: insights into structure, Codon usage, repeats, and RNA editing.BMC genomics · 2023Article
- Detection and Analysis of C-to-U RNA Editing in Rice Mitochondria-Encoded ORFs.Plants (Basel, Switzerland) · 2020Article
- Isolation of Intact Chloroplast for Sequencing Plastid Genomes of FivePlants (Basel, Switzerland) · 2019Article
- Transcriptome analysis of the model grass Lolium temulentum exposed to green leaf volatiles.BMC plant biology · 2019Article
- The comparison of four mitochondrial genomes reveals cytoplasmic male sterility candidate genes in cotton.BMC genomics · 2018Article
- NaOH low-salt method for chloroplast isolation and highly pure cpDNA preparation fromApplications in plant sciences · 2018Article
- The landscape of mitochondrial small non-coding RNAs in the PGCs of male mice, spermatogonia, gametes and in zygotes.BMC genomics · 2018Article
- Towards a comprehensive picture of C-to-U RNA editing sites in angiosperm mitochondria.Plant molecular biology · 2018Article
- Whole-genome sequencing of Brassica oleracea var. capitata reveals new diversity of the mitogenome.PloS one · 2018Article
- A Dynamic Mobile DNA Family in the Yeast Mitochondrial Genome.G3 (Bethesda, Md.) · 2015Article
- The application of RNA-seq to the comprehensive analysis of plant mitochondrial transcriptomes.Molecular genetics and genomics : MGG · 2015Review
- Genome-Wide Transcriptional Profiling and Metabolic Analysis Uncover Multiple Molecular Responses of the Grass Species Lolium perenne Under Low-Intensity Xenobiotic Stress.Frontiers in plant science · 2015Article
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPerennial ryegrass (Lolium perenne L.) is one of the most important forage and turf grass species of temperate regions worldwide. Its mitochondrial genome is inherited maternally and contains genes that can influence traits of agricultural importance. Moreover, the DNA sequence of mitochondrial genomes has been established and compared for a large number of species in order to characterize evolutionary relationships. Therefore, it is crucial to understand the organization of the mitochondrial genome and how it varies between and within species. Here, we report the first de novo assembly and annotation of the complete mitochondrial genome from perennial ryegrass.
resultsIntact mitochondria from perennial ryegrass leaves were isolated and used for mtDNA extraction. The mitochondrial genome was sequenced to a 167-fold coverage using the Roche 454 GS-FLX Titanium platform, and assembled into a circular master molecule of 678,580 bp. A total of 34 proteins, 14 tRNAs and 3 rRNAs are encoded by the mitochondrial genome, giving a total gene space of 48,723 bp (7.2%). Moreover, we identified 149 open reading frames larger than 300 bp and covering 67,410 bp (9.93%), 250 SSRs, 29 tandem repeats, 5 pairs of large repeats, and 96 pairs of short inverted repeats. The genes encoding subunits of the respiratory complexes - nad1 to nad9, cob, cox1 to cox3 and atp1 to atp9 - all showed high expression levels both in absolute numbers and after normalization.
conclusionsThe circular master molecule of the mitochondrial genome from perennial ryegrass presented here constitutes an important tool for future attempts to compare mitochondrial genomes within and between grass species. Our results also demonstrate that mitochondria of perennial ryegrass contain genes crucial for energy production that are well conserved in the mitochondrial genome of monocotyledonous species. The expression analysis gave us first insights into the transcriptome of these mitochondrial genes in perennial ryegrass.
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