ArticlePlant physiology2013
A guanine nucleotide exchange factor for Rab5 proteins is essential for intracellular transport of the proglutelin from the Golgi apparatus to the protein storage vacuole in rice endosperm.
Article in Plant physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 63 citations in OpenAlex.
- Natural Variation and Expression of OsCFF1 Affect the Formation of Rice Chalkiness.Rice (New York, N.Y.) · 2026Article
- Integrative Transcriptomic and Phosphoproteomic Analysis Reveals Key Components of the SnRK1 Signaling Network in Rice.Plant direct · 2025Article
- The MON1-CCZ1 complex plays dual roles in autophagic degradation and vacuolar protein transport in rice.Journal of integrative plant biology · 2025Article
- Involvement of a rice mutation in storage protein biogenesis in endosperm and its genomic location.Planta · 2024Article
- The Arabidopsis Rab protein RABC1 affects stomatal development by regulating lipid droplet dynamics.The Plant cell · 2022Article
- Article
- Dynamic formation and transcriptional regulation mediated by phytohormones during chalkiness formation in rice.BMC plant biology · 2021Article
- Melatonin Participates in Selenium-Enhanced Cold Tolerance of Cucumber Seedlings.Frontiers in plant science · 2021Article
- Recent Advances in Carbon and Nitrogen Metabolism in C3 Plants.International journal of molecular sciences · 2020Review
- Article
- OsNHX5-mediated pH homeostasis is required for post-Golgi trafficking of seed storage proteins in rice endosperm cells.BMC plant biology · 2019Article
- Glutelin subtype-dependent protein localization in rice grain evidenced by immunodetection analyses.Plant molecular biology · 2019Article
- A conserved motif is essential for the correct assembly of proglutelins and for their export from the endoplasmic reticulum in rice endosperm.Journal of experimental botany · 2018Article
- Selective sets of mRNAs localize to extracellular paramural bodies in a rice glup6 mutant.Journal of experimental botany · 2018Article
- SCD1 and SCD2 Form a Complex That Functions with the Exocyst and RabE1 in Exocytosis and Cytokinesis.The Plant cell · 2017Article
- Complementary Proteome and Transcriptome Profiling in Developing Grains of a Notched-Belly Rice Mutant Reveals Key Pathways Involved in Chalkiness Formation.Plant & cell physiology · 2017Article
- GOLGI TRANSPORT 1B Regulates Protein Export from the Endoplasmic Reticulum in Rice Endosperm Cells.The Plant cell · 2016Article
- Functional analyses of the plant-specific C-terminal region of VPS9a: the activating factor for RAB5 in Arabidopsis thaliana.Journal of plant research · 2016Article
- Golgi/plastid-type manganese superoxide dismutase involved in heat-stress tolerance during grain filling of rice.Plant biotechnology journal · 2015Article
- Guanine nucleotide exchange factor 2 for Rab5 proteins coordinated with GLUP6/GEF regulates the intracellular transport of the proglutelin from the Golgi apparatus to the protein storage vacuole in rice endosperm.Journal of experimental botany · 2015Article
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Authors and funding
14 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rice (Oryza sativa) glutelins are synthesized on the endoplasmic reticulum as a precursor, which are then transported via the Golgi to protein storage vacuoles (PSVs), where they are proteolytically processed into acidic and basic subunits. The glutelin precursor mutant6 (glup6) accumulates abnormally large amounts of proglutelin. Map-base cloning studies showed that glup6 was a loss-of-function mutant of guanine nucleotide exchange factor (GEF), which activates Rab GTPase, a key regulator of membrane trafficking. Immunofluorescence studies showed that the transport of proglutelins and α-globulins to PSV was disrupted in glup6 endosperm. Secreted granules of glutelin and α-globulin were readily observed in young glup6 endosperm, followed by the formation of large dilated paramural bodies (PMBs) containing both proteins as the endosperm matures. The PMBs also contained membrane biomarkers for the Golgi and prevacuolar compartment as well as the cell wall component, β-glucan. Direct evidence was gathered showing that GLUP6/GEF activated in vitro GLUP4/Rab5 as well as several Arabidopsis (Arabidopsis thaliana) Rab5 isoforms to the GTP-bound form. Therefore, loss-of-function mutations in GEF or Rab5 disrupt the normal transport of proglutelin from the Golgi to PSVs, resulting in the initial extracellular secretion of these proteins followed, in turn, by the formation of PMBs. Overall, our results indicate that GLUP6/GEF is the activator of Rab5 GTPase and that the cycling of GTP- and GDP-bound forms of this regulatory protein is essential for the intracellular transport of proglutelin and α-globulin from the Golgi to PSVs and in the maintenance of the general structural organization of the endomembrane system in rice seeds.
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