ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2013
cAMP-mediated stabilization of fusion pores in cultured rat pituitary lactotrophs.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Aluminum Exposure Impairs Electrochemically Measured Vesicular Storage and Exocytosis via Iron Homeostasis Dysregulation.Angewandte Chemie (International ed. in English) · 2025Article
- Ca2+ and cAMP open differentially dilating synaptic fusion pores.Journal of cell science · 2023Article
- PACAP and acetylcholine cause distinct Ca2+ signals and secretory responses in chromaffin cells.The Journal of general physiology · 2023Article
- Single-Vesicle Electrochemistry Following Repetitive Stimulation Reveals a Mechanism for Plasticity Changes with Iron Deficiency.Angewandte Chemie (International ed. in English) · 2022Article
- Nano-analysis Reveals High Fraction of Serotonin Release during Exocytosis from a Gut Epithelium Model Cell.Angewandte Chemie (International ed. in English) · 2021Article
- Astroglial Mechanisms of Ketamine Action Include Reduced Mobility of Kir4.1-Carrying Vesicles.Neurochemical research · 2020Review
- Astrocyte Specific Remodeling of Plasmalemmal Cholesterol Composition by Ketamine Indicates a New Mechanism of Antidepressant Action.Scientific reports · 2019Article
- Article
- Gliocrine System: Astroglia as Secretory Cells of the CNS.Advances in experimental medicine and biology · 2019Review
- Astroglial Vesicular Trafficking in Neurodegenerative Diseases.Neurochemical research · 2017Article
- Astrocytic Pathological Calcium Homeostasis and Impaired Vesicle Trafficking in Neurodegeneration.International journal of molecular sciences · 2017Review
- Review
- Calcium signaling properties of a thyrotroph cell line, mouse TαT1 cells.Cell calcium · 2015Article
- Excitable Astrocytes: Ca(2+)- and cAMP-Regulated Exocytosis.Neurochemical research · 2015Review
- Modeling of glucose-induced cAMP oscillations in pancreatic β cells: cAMP rocks when metabolism rolls.Biophysical journal · 2015Article
- Paliperidone and aripiprazole differentially affect the strength of calcium-secretion coupling in female pituitary lactotrophs.Scientific reports · 2015Article
- Serotonin-secreting enteroendocrine cells respond via diverse mechanisms to acute and chronic changes in glucose availability.Nutrition & metabolism · 2015Article
- Local electrostatic interactions determine the diameter of fusion pores.Channels (Austin, Tex.) · 2015Article
- Hyperpolarization-activated cyclic nucleotide-gated channels and cAMP-dependent modulation of exocytosis in cultured rat lactotrophs.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- The role of cyclic nucleotides in pituitary lactotroph functions.Frontiers in endocrinology · 2013Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 5 countries.
Funding
Abstract
Regulated exocytosis mediates the release of hormones and transmitters. The last step of this process is represented by the merger between the vesicle and the plasma membranes, and the formation of a fusion pore. Once formed, the initially stable and narrow fusion pore may reversibly widen (transient exocytosis) or fully open (full-fusion exocytosis). Exocytosis is typically triggered by an elevation in cytosolic calcium activity. However, other second messengers, such as cAMP, have been reported to modulate secretion. The way in which cAMP influences the transitions between different fusion pore states remains unclear. Here, hormone release studies show that prolactin release from isolated rat lactotrophs stimulated by forskolin, an activator of adenylyl cyclases, and by membrane-permeable cAMP analog (dbcAMP), exhibit a biphasic concentration dependency. Although at lower concentrations (2-10 μm forskolin and 2.5-5 mm dbcAMP) these agents stimulate prolactin release, an inhibition is measured at higher concentrations (50 μm forskolin and 10-15 mm dbcAMP). By using high-resolution capacitance (Cm) measurements, we recorded discrete increases in Cm, which represent elementary exocytic events. An elevation of cAMP leaves the frequency of full-fusion events unchanged while increasing the frequency of transient events. These exhibited a wider fusion pore as measured by increased fusion pore conductance and a prolonged fusion pore dwell time. The probability of observing rhythmic reopening of transient fusion pores was elevated by dbcAMP. In conclusion, cAMP-mediated stabilization of wide fusion pores prevents vesicles from proceeding to the full-fusion stage of exocytosis, which hinders vesicle content discharge at high cAMP concentrations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.