ArticleJournal of experimental botany2013
ARA7(Q69L) expression in transgenic Arabidopsis cells induces the formation of enlarged multivesicular bodies.
Article in Journal of experimental botany, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
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- The plant-unique protein DRIF1 coordinates with sorting nexin 1 to regulate membrane protein homeostasis.The Plant cell · 2023Article
- Rab GTPases, tethers, and SNAREs work together to regulateFrontiers in plant science · 2023Review
- Immunity functions of Arabidopsis pathogenesis-related 1 are coupled but not confined to its C-terminus processing and trafficking.Molecular plant pathology · 2022Article
- Turnip mosaic virus co-opts the vacuolar sorting receptor VSR4 to promote viral genome replication in plants by targeting viral replication vesicles to the endosome.PLoS pathogens · 2022Article
- Systematic analysis of specific and nonspecific auxin effects on endocytosis and trafficking.Plant physiology · 2021Article
- Dynamic membranes: the multiple roles of P4 and P5 ATPases.Plant physiology · 2021Article
- Organelle Visualization With Multicolored Fluorescent Markers in Bamboo.Frontiers in plant science · 2021Article
- Plant Cells under Attack: Unconventional Endomembrane Trafficking during Plant Defense.Plants (Basel, Switzerland) · 2020Review
- A Conditional Mutation inFrontiers in plant science · 2020Article
- A Diverse Membrane Interaction Network for Plant Multivesicular Bodies: Roles in Proteins Vacuolar Delivery and Unconventional Secretion.Frontiers in plant science · 2020Review
- ESCRT-dependent vacuolar sorting and degradation of the auxin biosynthetic enzyme YUC1 flavin monooxygenase.Journal of integrative plant biology · 2019Article
- Multiple downy mildew effectors target the stress-related NAC transcription factor LsNAC069 in lettuce.The Plant journal : for cell and molecular biology · 2019Article
- RST1 Is a FREE1 Suppressor That Negatively Regulates Vacuolar Trafficking in Arabidopsis.The Plant cell · 2019Article
- The Arabidopsis receptor kinase STRUBBELIG undergoes clathrin-dependent endocytosis.Journal of experimental botany · 2019Article
- Identification of Novel Inhibitors of Auxin-Induced CaPlant physiology · 2019Article
- Turnip Mosaic Virus Uses the SNARE Protein VTI11 in an Unconventional Route for Replication Vesicle Trafficking.The Plant cell · 2018Article
- The Arabidopsis USL1 controls multiple aspects of development by affecting late endosome morphology.The New phytologist · 2018Article
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9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Arabidopsis thaliana ARA7 (AtRabF2b), a member of the plant Rab5 small GTPases functioning in the vacuolar transport pathway, localizes to pre-vacuolar compartments (PVCs), known as multivesicular bodies (MVBs) in plant cells. Overexpression of the constitutively active GTP-bound mutant of ARA7, ARA7(Q69L), induces the formation of large ring-like structures (1-2 µm in diameter). To better understand the biology of these ARA7(Q69L)-induced ring-like structures, transgenic Arabidopsis cell lines expressing ARA7(Q69L) tagged with green fluorescent protein (GFP) under the control of a heat shock-inducible promoter were generated. In these transgenic cells, robust ring-like structures were formed after 4 h of heat shock induction. Transient co-expression, confocal imaging, and immunogold electron microscopy (immunogold-EM) experiments demonstrated that these GFP-ARA7(Q69L)-labelled ring-like structures were distinct from the Golgi apparatus and trans-Golgi network, but were labelled with an antibody against an MVB marker protein. In addition, live cell imaging and detailed EM analysis showed that the GFP-ARA7(Q69L)-induced spherical structures originated from the homotypic fusion of MVBs. In summary, it was demonstrated that GFP-ARA7(Q69L) expression is an efficient tool for studying PVC/MVB-mediated protein trafficking and vacuolar degradation in plant cells.
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