Evidence map›Paper›PMID 23885017›Full record

ArticleEndocrinology2013

ROCK1 in AgRP neurons regulates energy expenditure and locomotor activity in male mice.

Hu Huang, Seung Hwan Lee, Chianping Ye, Ines S Lima, Byung-Chul Oh, Bradford B Lowell, Janice M Zabolotny, Young-Bum Kim

Open access · bronzeAbstract read
In one paragraph

Article in Endocrinology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed
1.6field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 43 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Article
  5. Hypothalamic control of energy expenditure and thermogenesis.Experimental & molecular medicine · 2022
    Review
  6. Review
  7. Review
  8. Review
  9. Article
  10. Review
  11. ARCFrontiers in physiology · 2020
    Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Hypothalamic control of brown adipose tissue thermogenesis.Frontiers in systems neuroscience · 2015
    Review
  19. Article
  20. ROCK1 isoform-specific deletion reveals a role for diet-induced insulin resistance.American journal of physiology. Endocrinology and metabolism · 2014
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 3 countries.

Hu HuangPhD, Division of Endocrinology, Diabetes, and Metabolism, Beth Israel Deaconess Medical Center, 330 Brookline Avenue, Boston, Massachusetts 02215. ykim2@bidmc.harvard.edu.
Seung Hwan Lee
Chianping Ye
Ines S Lima
Byung-Chul Oh
Bradford B Lowell
Janice M Zabolotny
Young-Bum Kim
Harvard University · USBeth Israel Deaconess Medical Center · USGachon University · KRUniversidade Nova de Lisboa · PT

Funding

AGRP NEURONS. NMDARs, Spines, Source of Excitatory Input and Downstream EffectorsR01DK096010 · NIDDK · BETH ISRAEL DEACONESS MEDICAL CENTER · PI LOWELL, BRADFORD B · 2012 to 2024
$7.2M
Neurocircuitry Underlying Leptin Regulation of Energy BalanceR01DK089044 · NIDDK · BETH ISRAEL DEACONESS MEDICAL CENTER · PI LOWELL, BRADFORD B · 2010 to 2023
$6.0M
ROCK1 Signaling in Glucose MetabolismR01DK083567 · NIDDK · BETH ISRAEL DEACONESS MEDICAL CENTER · PI KIM, YOUNG-BUM · 2010 to 2018
$3.4M
NIDDK NIH HHS 1R01DK083567NIDDK NIH HHS R01 DK083567NIDDK NIH HHS R01 DK089044NIDDK NIH HHS R01 DK096010
6 · The paper itself

Abstract

Normal leptin signaling is essential for the maintenance of body weight homeostasis. Proopiomelanocortin- and agouti-related peptide (AgRP)-producing neurons play critical roles in regulating energy metabolism. Our recent work demonstrates that deletion of Rho-kinase 1 (ROCK1) in the AgRP neurons of mice increased body weight and adiposity. Here, we report that selective loss of ROCK1 in AgRP neurons caused a significant decrease in energy expenditure and locomotor activity of mice. These effects were independent of any change in food intake. Furthermore, AgRP neuron-specific ROCK1-deficient mice displayed central leptin resistance, as evidenced by impaired Signal Transducer and Activator of Transcription 3 activation in response to leptin administration. Leptin's ability to hyperpolarize and decrease firing rate of AgRP neurons was also abolished in the absence of ROCK1. Moreover, diet-induced and genetic forms of obesity resulted in reduced ROCK1 activity in murine arcuate nucleus. Of note, high-fat diet also impaired leptin-stimulated ROCK1 activity in arcuate nucleus, suggesting that a defect in hypothalamic ROCK1 activity may contribute to the pathogenesis of central leptin resistance in obesity. Together, these data demonstrate that ROCK1 activation in hypothalamic AgRP neurons is required for the homeostatic regulation of energy expenditure and adiposity. These results further support previous work identifying ROCK1 as a key regulator of energy balance and suggest that targeting ROCK1 in the hypothalamus may lead to development of antiobesity therapeutics.

Indexed as

Energy MetabolismMotor ActivityAgouti-Related ProteinAnimalsArcuate Nucleus of HypothalamusBehavior, AnimalCrosses, GeneticEnergy IntakeLeptinMaleMiceMice, Inbred C57BLMice, KnockoutMice, TransgenicNerve Tissue ProteinsNeuronsagouti-related peptide, (Yc(CRFFNAFC)Y)Agouti-Related ProteinAgrp protein, mouseLeptinNerve Tissue ProteinsPeptide FragmentsRecombinant Fusion Proteinsrho-Associated KinasesRock1 protein, mouseStat3 protein, mouseSTAT3 Transcription Factor

Identifiers

PMID23885017
PMCPMC3776869
OpenAlexW2059292612

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.