ArticleCirculation. Cardiovascular genetics2013
Natural cardiogenesis-based template predicts cardiogenic potential of induced pluripotent stem cell lines.
Article in Circulation. Cardiovascular genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 18 citations in OpenAlex.
- Uncoupling of Proliferative Capacity from Developmental Stage During Directed Cardiac Differentiation of Pluripotent Stem Cells.Stem cells and development · 2022Article
- CXCL4/PF4 is a predictive biomarker of cardiac differentiation potential of human induced pluripotent stem cells.Scientific reports · 2019Article
- The Genetic Landscape of Hypoplastic Left Heart Syndrome.Pediatric cardiology · 2018Article
- The complex genetics of hypoplastic left heart syndrome.Nature genetics · 2017Article
- Systems-based technologies in profiling the stem cell molecular framework for cardioregenerative medicine.Stem cell reviews and reports · 2015Review
- Identification of cardiovascular lineage descendants at single-cell resolution.Development (Cambridge, England) · 2015Article
- Induced pluripotent stem cells for cardiovascular disease: from product-focused disease modeling to process-focused disease discovery.Regenerative medicine · 2015Review
- Selection via pluripotency-related transcriptional screen minimizes the influence of somatic origin on iPSC differentiation propensity.Stem cells (Dayton, Ohio) · 2014Article
- Comparison of reprogramming genes in induced pluripotent stem cells and nuclear transfer cloned embryos.Stem cell reviews and reports · 2014Article
- Rbm20-deficient cardiogenesis reveals early disruption of RNA processing and sarcomere remodeling establishing a developmental etiology for dilated cardiomyopathy.Human molecular genetics · 2014Article
- Transcriptional atlas of cardiogenesis maps congenital heart disease interactome.Physiological genomics · 2014Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundCardiac development is a complex process resulting in an integrated, multilineage tissue with developmental corruption in early embryogenesis leading to congenital heart disease. Interrogation of individual genes has provided the backbone for cardiac developmental biology, yet a comprehensive transcriptome derived from natural cardiogenesis is required to gauge innate developmental milestones. METHODS AND
resultsStage-specific cardiac structures were dissected from 8 distinctive mouse embryonic time points to produce genome-wide expressome analysis across cardiogenesis. With reference to this native cardiogenic expression roadmap, divergent induced pluripotent stem cell-derived cardiac expression profiles were mapped from procardiogenic 3-factor (SOX2, OCT4, KLF4) and less-cardiogenic 4-factor (plus c-MYC) reprogrammed cells. Expression of cardiac-related genes from 3-factor-induced pluripotent stem cell differentiated in vitro at days 5 and 11 and recapitulated expression profiles of natural embryos at days E7.5-E8.5 and E14.5-E18.5, respectively. By contrast, 4-factor-induced pluripotent stem cells demonstrated incomplete cardiogenic gene expression profiles beginning at day 5 of differentiation. Differential gene expression within the pluripotent state revealed 23 distinguishing candidate genes among pluripotent cell lines with divergent cardiogenic potentials. A confirmed panel of 12 genes, differentially expressed between high and low cardiogenic lines, was transformed into a predictive score sufficient to discriminate individual induced pluripotent stem cell lines according to relative cardiogenic potential.
conclusionsTranscriptome analysis attuned to natural embryonic cardiogenesis provides a robust platform to probe coordinated cardiac specification and maturation from bioengineered stem cell-based model systems. A panel of developmental-related genes allowed differential prognosis of cardiogenic competency, thus prioritizing cell lines according to natural blueprint to streamline functional applications.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.