ArticleMolecular and cellular biology1990
Diacylglycerol production in Xenopus laevis oocytes after microinjection of p21ras proteins is a consequence of activation of phosphatidylcholine metabolism.
Article in Molecular and cellular biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 72 citations in OpenAlex.
- Metabolic effects of signal transduction inhibition in cancer assessed by magnetic resonance spectroscopy.Molecular oncology · 2011Review
- Regulation of Akt(ser473) phosphorylation by choline kinase in breast carcinoma cells.Molecular cancer · 2009Article
- Magnetic resonance detects changes in phosphocholine associated with Ras activation and inhibition in NIH 3T3 cells.British journal of cancer · 2001Article
- Increased choline kinase activity in 1,2-dimethylhydrazine-induced rat colon cancer.Japanese journal of cancer research : Gann · 1999Article
- Increased choline kinase activity and elevated phosphocholine levels in human colon cancer.Japanese journal of cancer research : Gann · 1999Article
- Activation of intracellular kinases in Xenopus oocytes by p21ras and phospholipases: a comparative study.Molecular and cellular biology · 1995Article
- Protein turnover in 3T3 cells transformed with the oncogene c-H-ras1.The Biochemical journal · 1992Article
- Testing the in vivo role of protein kinase C and c-fos in neurite outgrowth by microinjection of antibodies into PC12 cells.Molecular biology of the cell · 1992Article
- The biochemistry of ras p21.The Biochemical journal · 1991Review
- v-Src increases diacylglycerol levels via a type D phospholipase-mediated hydrolysis of phosphatidylcholine.Molecular and cellular biology · 1991Article
- The mitogenic activities of phosphatidate are acyl-chain-length dependent and calcium independent in C3H/10T1/2 cells.Cell regulation · 1991Article
- Increased conversion of phosphatidylinositol to phosphatidylinositol phosphate in Dictyostelium cells expressing a mutated ras gene.Proceedings of the National Academy of Sciences of the United States of America · 1990Article
- H-ras(val12) induces cytoplasmic but not nuclear events of the cell cycle in small Xenopus oocytes.Cell regulation · 1990Article
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Authors and funding
1 author.
Funding
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Abstract
Microinjection of p21Ha-ras proteins into Xenopus laevis oocytes induces a rapid increase of 1,2-diacylglycerol (DAG) levels. The observed alterations in DAG levels were consistent with the ability of the protein to induce maturation, measured by germinal vesicle breakdown (GVBD). Both the increase in DAG levels and GVBD activity were dependent on the ability of the proteins to undergo membrane translocation. Alterations of DAG levels or GVBD activity did not correlate with changes in the levels of inositol phosphates. However, at minimal doses sufficient to achieve maximal biological response, a biphasic increase in the amounts of phosphocholine and CDP-choline was observed. The first burst of phosphocholine and CDP-choline preceded the increase in DAG levels. The second peak paralleled the appearance of DAG. Choline kinase activity was also increased in oocyte extracts after p21ras microinjection. These results suggest that both the synthesis and degradation of phosphatidylcholine are activated after microinjection of ras proteins into Xenopus oocytes, resulting in a net production of DAG.
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