Evidence map›Paper›PMID 24101677›Full record

ArticleDiabetes2014

Podocyte-specific GLUT4-deficient mice have fewer and larger podocytes and are protected from diabetic nephropathy.

Johanna Guzman, Alexandra N Jauregui, Sandra Merscher-Gomez, Dony Maiguel, Cristina Muresan, Alla Mitrofanova, Ana Diez-Sampedro, Joel Szust, Tae-Hyun Yoo, Rodrigo Villarreal and 11 more

Open access · bronzeAbstract read
In one paragraph

Article in Diabetes, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed
2.8field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

32 citing papers in PubMed, 58 citations in OpenAlex.

  1. Article
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  7. Review
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  9. Review
  10. Article
  11. Article
  12. Critical Role for AMPK in Metabolic Disease-Induced Chronic Kidney Disease.International journal of molecular sciences · 2020
    Review
  13. Article
  14. Podocyte Lysosome Dysfunction in Chronic Glomerular Diseases.International journal of molecular sciences · 2020
    Review
  15. Yu Nu Compound Regulates Autophagy and Apoptosis Through mTOR in vivo and vitro.Diabetes, metabolic syndrome and obesity : targets and therapy · 2020
    Article
  16. Lipid mediators of insulin signaling in diabetic kidney disease.American journal of physiology. Renal physiology · 2019
    Review
  17. Article
  18. Article
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors at 5 institutions in 2 countries.

Johanna GuzmanDiabetes Research Institute, Miller School of Medicine, University of Miami, Miami, FL.
Alexandra N Jauregui
Sandra Merscher-Gomez
Dony Maiguel
Cristina Muresan
Alla Mitrofanova
Ana Diez-Sampedro
Joel Szust
Tae-Hyun Yoo
Rodrigo Villarreal
Christopher Pedigo
R Damaris Molano
Kevin Johnson
Barbara Kahn
Bjoern Hartleben
Tobias B Huber
Jharna Saha
George W Burke
E Dale Abel
Frank C Brosius
Alessia Fornoni
University of Miami · USUniversity of Freiburg · DEUniversity of Michigan–Ann Arbor · USBeth Israel Deaconess Medical Center · USUniversity of Utah · US

Funding

Regional Pilot And Feasibility Study Grants ProgramP30DK020572 · NIDDK · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI DAVID P OLSON · 2013 to 2026
$24.3M
Miami Clinical and Translational Science InstituteUL1TR000460 · NCATS · UNIVERSITY OF MIAMI SCHOOL OF MEDICINE · PI SACCO, RALPH L. · 2012 to 2016
$16.9M
Research BaseP30DK092926 · NIDDK · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI MARY ELLEN MICHELE HEISLER, ADESUWA B OLOMU · 2011 to 2026
$10.0M
Glucose Transporter Regulation in Obesity and DiabetesR37DK043051 · NIDDK · BETH ISRAEL DEACONESS MEDICAL CENTER · PI KAHN, BARBARA B. · 2006 to 2015
$5.0M
GLUCOSE TRANSPORTER REGULATION IN OBESITY AND DIABETESR01DK043051 · NIDDK · BETH ISRAEL DEACONESS MEDICAL CENTER · PI KAHN, BARBARA B. · 1992 to 2020
$4.4M
Modeling Diabetic Cardiomyopathy and Microangiopathy U01HL087947 · NHLBI · UNIVERSITY OF UTAH · PI ABEL, E DALE · 2006 to 2010
$1.8M
Novel Targets of Rituximab in FSGSR01DK090316 · NIDDK · UNIVERSITY OF MIAMI SCHOOL OF MEDICINE · PI FORNONI, ALESSIA · 2011 to 2015
$1.7M
NCATS NIH HHS UL1 TR000460NHLBI NIH HHS U01 HL087947NIDDK NIH HHS P30 DK020572NIDDK NIH HHS P30 DK092926NIDDK NIH HHS R01 DK043051NIDDK NIH HHS R01 DK090316NIDDK NIH HHS R37 DK043051
6 · The paper itself

Abstract

Podocytes are a major component of the glomerular filtration barrier, and their ability to sense insulin is essential to prevent proteinuria. Here we identify the insulin downstream effector GLUT4 as a key modulator of podocyte function in diabetic nephropathy (DN). Mice with a podocyte-specific deletion of GLUT4 (G4 KO) did not develop albuminuria despite having larger and fewer podocytes than wild-type (WT) mice. Glomeruli from G4 KO mice were protected from diabetes-induced hypertrophy, mesangial expansion, and albuminuria and failed to activate the mammalian target of rapamycin (mTOR) pathway. In order to investigate whether the protection observed in G4 KO mice was due to the failure to activate mTOR, we used three independent in vivo experiments. G4 KO mice did not develop lipopolysaccharide-induced albuminuria, which requires mTOR activation. On the contrary, G4 KO mice as well as WT mice treated with the mTOR inhibitor rapamycin developed worse adriamycin-induced nephropathy than WT mice, consistent with the fact that adriamycin toxicity is augmented by mTOR inhibition. In summary, GLUT4 deficiency in podocytes affects podocyte nutrient sensing, results in fewer and larger cells, and protects mice from the development of DN. This is the first evidence that podocyte hypertrophy concomitant with podocytopenia may be associated with protection from proteinuria.

Indexed as

AlbuminuriaAnimalsCell SizeDiabetic NephropathiesDoxorubicinFemaleGene Expression RegulationGlomerular Filtration BarrierGlucose Transporter Type 1Glucose Transporter Type 4LipopolysaccharidesMicePodocytesDoxorubicinGlucose Transporter Type 1Glucose Transporter Type 4LipopolysaccharidesSlc2a1 protein, mouseSlc2a4 protein, mouse

Identifiers

PMID24101677
PMCPMC3900538
OpenAlexW2122807157

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.