ArticleThe Plant cell2013
Identification of downstream components of ubiquitin-conjugating enzyme PHOSPHATE2 by quantitative membrane proteomics in Arabidopsis roots.
Article in The Plant cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 115 papers.
What it found
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Who cites it
115 citing papers in PubMed, 278 citations in OpenAlex.
- CsSPX-PHR/PHL-PHT1 and CsmiR399a-PHO2-PHT1/PHO1 Modules Orchestrate Pi Use Efficiency and Aluminium Tolerance in Tea Plants.Plant, cell & environment · 2026Article
- The PHO1 protein family in phosphate export and beyond.Plant physiology · 2026Review
- PHR-Mediated Pi Starvation Response Mobile Messenger RNAs Represent Noncoding Transcripts in Recipient Tissues.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Cargo Sorting Zones in Plant Trans-Golgi Network.Sub-cellular biochemistry · 2026Review
- Beyond phosphate uptake: PT4/PT11 as key functional components linking phosphate acquisition, nutrient exchange, and arbuscule maintenance in arbuscular mycorrhizal symbiosis.Frontiers in plant science · 2026Review
- Overexpression of miR399d impairs arbuscular mycorrhizal symbiosis in tomato.Plant biotechnology journal · 2025Article
- Effects of suppression of phosphate transporter 4;4 on COJournal of plant research · 2025Article
- The Impact of Nitrogen and Phosphorus Interaction on Growth, Nutrient Absorption, and Signal Regulation in Woody Plants.Biology · 2025Article
- Sink strength, nutrient allocation, cannabinoid yield, and associated transcript profiles vary in two drug-type Cannabis chemovars.Journal of experimental botany · 2025Article
- Mobilization and recycling of intracellular phosphorus in response to availability.Quantitative plant biology · 2025Review
- From genes to traits: maximizing phosphorus utilization efficiency in crop plants.Frontiers in plant science · 2025Review
- Plants Under Stress: Exploring Physiological and Molecular Responses to Nitrogen and Phosphorus Deficiency.Plants (Basel, Switzerland) · 2024Review
- Insights into maydis leaf blight resistance in maize: a comprehensive genome-wide association study in sub-tropics of India.BMC genomics · 2024Article
- Mining for QTL controlling maize low-phosphorus response genes combined with deep resequencing of RIL parental genomes and in silico GWAS analysis.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Untranslated yet indispensable-UTRs act as key regulators in the environmental control of gene expression.Journal of experimental botany · 2024Review
- Exploring the phosphorus-starch content balance mechanisms in maize grains using GWAS population and transcriptome data.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Milestones in understanding transport, sensing, and signaling of the plant nutrient phosphorus.The Plant cell · 2024Review
- Modulation of plant immunity and biotic interactions under phosphate deficiency.Journal of plant research · 2024Review
- Recent advances in research on phosphate starvation signaling in plants.Journal of plant research · 2024Review
- Visualizing plant intracellular inorganic orthophosphate distribution.Nature plants · 2024Article
55 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
13 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNA399-mediated regulation of the ubiquitin-conjugating enzyme UBC24/phosphate2 (PHO2) is crucial for Pi acquisition and translocation in plants. Because of a potential role for PHO2 in protein degradation and its association with membranes, an iTRAQ (for isobaric tags for relative and absolute quantitation)- based quantitative membrane proteomic method was employed to search for components downstream of PHO2. A total of 7491 proteins were identified from Arabidopsis thaliana roots by mass spectrometry, 35.2% of which were predicted to contain at least one transmembrane helix. Among the quantifiable proteins, five were significantly differentially expressed between the wild type and pho2 mutant under two growth conditions. Using immunoblot analysis, we validated the upregulation of several members in phosphate transporter1 (PHT1) family and phosphate transporter traffic facilitator1 (PHF1) in pho2 and demonstrated that PHO2 mediates the degradation of PHT1 proteins. Genetic evidence that loss of PHF1 or PHT1;1 alleviated Pi toxicity in pho2 further suggests that they play roles as downstream components of PHO2. Moreover, we showed that PHO2 interacts with PHT1s in the postendoplasmic reticulum compartments and mediates the ubiquitination of endomembrane-localized PHT1;1. This study not only uncovers a mechanism by which PHO2 modulates Pi acquisition by regulating the abundance of PHT1s in the secretory pathway destined for plasma membranes, but also provides a database of the membrane proteome that will be widely applicable in root biology research.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.