ArticlePloS one2013
Proteomics analysis of alfalfa response to heat stress.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
37 citing papers in PubMed, 89 citations in OpenAlex.
- Thermal priming enhances heat tolerance in alfalfa (Medicago sativa L.) through activation of multiple metabolic pathways.BMC plant biology · 2025Article
- Plant Hormone Stimulation andPlants (Basel, Switzerland) · 2025Article
- Changes in proteome and protein carbonylation in potato (Solanum tuberosum L.) under single and combined abiotic stresses.Scientific reports · 2025Article
- Harnessing the Potential of CRISPR/Cas in Targeted Alfalfa Improvement for Stress Resilience.International journal of molecular sciences · 2025Review
- Evaluation of Salt-Tolerant Germplasms and Identification of Salt Tolerance-Related Proteins in Upland Cotton at the Seedling Stage.International journal of molecular sciences · 2025Article
- Tandem mass tag (TMT)-based quantitative proteomics analysis reveals the different responses of contrasting alfalfa varieties to drought stress.BMC genomics · 2024Article
- Mapping heat tolerance QTLs inFrontiers in plant science · 2024Article
- Grafting enhances drought tolerance by regulating and mobilizing proteome, transcriptome and molecular physiology in okra genotypes.Frontiers in plant science · 2023Article
- Responses of the tree peony (Paeonia suffruticosa, Paeoniaceae) cultivar 'Yu Hong' to heat stress revealed by iTRAQ-based quantitative proteomics.Proteome science · 2022Article
- Molecular insights into sensing, regulation and improving of heat tolerance in plants.Plant cell reports · 2022Review
- Genetic and Physiological Responses to Heat Stress inFrontiers in plant science · 2022Review
- Climate change regulated abiotic stress mechanisms in plants: a comprehensive review.Plant cell reports · 2022Review
- Label-free quantitative proteomic analysis of alfalfa in response to microRNA156 under high temperature.BMC genomics · 2020Article
- Superior adjuvanticity of the genetically fused D1 domain of Neisseria meningitides Ag473 lipoprotein among three Toll-like receptor ligands.Bioscience reports · 2020Article
- Biotechnological Perspectives of Omics and Genetic Engineering Methods in Alfalfa.Frontiers in plant science · 2020Review
- Heat-Responsive Proteomics of a Heat-Sensitive Spinach Variety.International journal of molecular sciences · 2019Article
- Comparative Proteomics and Physiological Analyses Reveal Important Maize Filling-Kernel Drought-Responsive Genes and Metabolic Pathways.International journal of molecular sciences · 2019Article
- Proteomic analysis reveals response of differential wheat (Triticum aestivum L.) genotypes to oxygen deficiency stress.BMC genomics · 2019Article
- A Strong Impact of Soil Tetracycline on Physiology and Biochemistry of Pea Seedlings.Scientifica · 2019Article
- Label-free quantitative proteomic analysis ofJournal of ginseng research · 2019Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The proteome responses to heat stress have not been well understood. In this study, alfalfa (Medicago sativa L. cv. Huaiyin) seedlings were exposed to 25 °C (control) and 40 °C (heat stress) in growth chambers, and leaves were collected at 24, 48 and 72 h after treatment, respectively. The morphological, physiological and proteomic processes were negatively affected under heat stress. Proteins were extracted and separated by two-dimensional polyacrylamide gel electrophoresis (2-DE), and differentially expressed protein spots were identified by mass spectrometry (MS). Totally, 81 differentially expressed proteins were identified successfully by MALDI-TOF/TOF. These proteins were categorized into nine classes: including metabolism, energy, protein synthesis, protein destination/storage, transporters, intracellular traffic, cell structure, signal transduction and disease/defence. Five proteins were further analyzed for mRNA levels. The results of the proteomics analyses provide a better understanding of the molecular basis of heat-stress responses in alfalfa.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.