ArticleBMC genomics2014
Deep sequencing of the tobacco mitochondrial transcriptome reveals expressed ORFs and numerous editing sites outside coding regions.
Article in BMC genomics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
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- Repeat-mediated recombination results in Complex DNA structure of the mitochondrial genome of Trachelospermum jasminoides.BMC plant biology · 2024Article
- Experimental approaches to studying translation in plant semi-autonomous organelles.Journal of experimental botany · 2024Review
- Freshwater mussels prefer a diet of stramenopiles and fungi over bacteria.Scientific reports · 2024Article
- Assembly and characterization of the complete mitochondrial genome of Ventilago leiocarpa.Plant cell reports · 2024Article
- Genetic diversity ofFrontiers in plant science · 2023Article
- Emergence of Novel RNA-Editing Sites by Changes in the Binding Affinity of a Conserved PPR Protein.Molecular biology and evolution · 2022Article
- The Mitogenome ofBiology · 2022Article
- Evolution of mitochondrial RNA editing in extant gymnosperms.The Plant journal : for cell and molecular biology · 2022Article
- Graph-based models of theNAR genomics and bioinformatics · 2022Article
- Mito-FUNCAT-FACS reveals cellular heterogeneity in mitochondrial translation.RNA (New York, N.Y.) · 2022Article
- Article
- RNA Editing and Its Roles in Plant Organelles.Frontiers in genetics · 2021Review
- Detection and Analysis of C-to-U RNA Editing in Rice Mitochondria-Encoded ORFs.Plants (Basel, Switzerland) · 2020Article
- Article
- Dynamic response of RNA editing to temperature in grape by RNA deep sequencing.Functional & integrative genomics · 2020Article
- Comparative RNA editing profile of mitochondrial transcripts in cytoplasmic male sterile and fertile pigeonpea reveal significant changes at the protein level.Molecular biology reports · 2019Article
- High Level of Conservation of Mitochondrial RNA Editing Sites Among FourG3 (Bethesda, Md.) · 2019Article
- Homologous recombination changes the context of Cytochrome b transcription in the mitochondrial genome of Silene vulgaris KRA.BMC genomics · 2018Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe purpose of this study was to sequence and assemble the tobacco mitochondrial transcriptome and obtain a genomic-level view of steady-state RNA abundance. Plant mitochondrial genomes have a small number of protein coding genes with large and variably sized intergenic spaces. In the tobacco mitogenome these intergenic spaces contain numerous open reading frames (ORFs) with no clear function.
resultsThe assembled transcriptome revealed distinct monocistronic and polycistronic transcripts along with large intergenic spaces with little to no detectable RNA. Eighteen of the 117 ORFs were found to have steady-state RNA amounts above background in both deep-sequencing and qRT-PCR experiments and ten of those were found to be polysome associated. In addition, the assembled transcriptome enabled a full mitogenome screen of RNA C→U editing sites. Six hundred and thirty five potential edits were found with 557 occurring within protein-coding genes, five in tRNA genes, and 73 in non-coding regions. These sites were found in every protein-coding transcript in the tobacco mitogenome.
conclusionThese results suggest that a small number of the ORFs within the tobacco mitogenome may produce functional proteins and that RNA editing occurs in coding and non-coding regions of mitochondrial transcripts.
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