Evidence map›Paper›PMID 24503134›Full record

ArticleJournal of lipid research2014

LipidSeq: a next-generation clinical resequencing panel for monogenic dyslipidemias.

Christopher T Johansen, Joseph B Dubé, Melissa N Loyzer, Austin MacDonald, David E Carter, Adam D McIntyre, Henian Cao, Jian Wang, John F Robinson, Robert A Hegele

Abstract read
In one paragraph

Article in Journal of lipid research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.

0numbers the graph read from it
0cells of the map it votes in
50citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

50 citing papers in PubMed.

  1. Trial
  2. Article
  3. Article
  4. Article
  5. Reduced lipoprotein (a) in patients with severe hypertriglyceridaemia.The Journal of international medical research · 2024
    Article
  6. Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Identification of novel variants in theBiomedical reports · 2021
    Article
  15. Article
  16. Review
  17. Article
  18. Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Christopher T JohansenRobarts Research Institute, Schulich School of Medicine and Dentistry, Western University, London, Ontario, Canada N6A 5B7.
Joseph B Dubé
Melissa N Loyzer
Austin MacDonald
David E Carter
Adam D McIntyre
Henian Cao
Jian Wang
John F Robinson
Robert A Hegele

Funding

Canadian Institutes of Health Research MOP-13430Canadian Institutes of Health Research MOP-79523
6 · The paper itself

Abstract

We report the design of a targeted resequencing panel for monogenic dyslipidemias, LipidSeq, for the purpose of replacing Sanger sequencing in the clinical detection of dyslipidemia-causing variants. We also evaluate the performance of the LipidSeq approach versus Sanger sequencing in 84 patients with a range of phenotypes including extreme blood lipid concentrations as well as additional dyslipidemias and related metabolic disorders. The panel performs well, with high concordance (95.2%) in samples with known mutations based on Sanger sequencing and a high detection rate (57.9%) of mutations likely to be causative for disease in samples not previously sequenced. Clinical implementation of LipidSeq has the potential to aid in the molecular diagnosis of patients with monogenic dyslipidemias with a high degree of speed and accuracy and at lower cost than either Sanger sequencing or whole exome sequencing. Furthermore, LipidSeq will help to provide a more focused picture of monogenic and polygenic contributors that underlie dyslipidemia while excluding the discovery of incidental pathogenic clinically actionable variants in nonmetabolism-related genes, such as oncogenes, that would otherwise be identified by a whole exome approach, thus minimizing potential ethical issues.

Indexed as

Molecular Diagnostic TechniquesDNA Mutational AnalysisDyslipidemiasGenetic Predisposition to DiseaseHigh-Throughput Nucleotide SequencingHumansMolecular Sequence AnnotationMutationDNA diagnosisfamilial dyslipidemiagenetic risk scoremutationsnext generation sequencingpolygenic dyslipidemiaSanger sequencing

Identifiers

PMID24503134
PMCPMC3966710

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.