Evidence map›Paper›PMID 24505376›Full record

ArticlePloS one2014

Effect of peripheral 5-HT on glucose and lipid metabolism in wether sheep.

Hitoshi Watanabe, Ryo Saito, Tatsuya Nakano, Hideyuki Takahashi, Yu Takahashi, Keisuke Sumiyoshi, Katsuyoshi Sato, Xiangning Chen, Natsumi Okada, Shunsuke Iwasaki and 8 more

Open access · goldAbstract read
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
8.2field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 38 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Article
  5. Article
  6. 5-HTInternational journal of molecular sciences · 2023
    Article
  7. Article
  8. Article
  9. Comparative Transcriptome Analyses ofFrontiers in microbiology · 2020
    Article
  10. Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors at 3 institutions in 2 countries.

Hitoshi WatanabeCellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Ryo SaitoCellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Tatsuya NakanoCellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Hideyuki TakahashiCellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Yu TakahashiCellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Keisuke SumiyoshiCellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Katsuyoshi SatoCellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Xiangning ChenCellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Natsumi OkadaCellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Shunsuke IwasakiCellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Dian W HarjantiDepartment of Animal Sciences, Faculty of Agriculture, Iwate University, Morioka, Japan.
Natsumi SekiguchiDepartment of Animal Sciences, Faculty of Agriculture, Iwate University, Morioka, Japan.
Hiroaki SanoDepartment of Animal Sciences, Faculty of Agriculture, Iwate University, Morioka, Japan.
Haruki KitazawaLaboratory of Food and Biomolecular Science, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Michael T RoseInstitute of Biological, Environmental and Rural Sciences, Aberystwyth University, Cardiganshire, United Kingdom.
Shyuichi OhwadaCellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Kouichi WatanabeCellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Hisashi AsoCellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Tohoku University · JPIwate University · JPAberystwyth University · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In mice, peripheral 5-HT induces an increase in the plasma concentrations of glucose, insulin and bile acids, and a decrease in plasma triglyceride, NEFA and cholesterol concentrations. However, given the unique characteristics of the metabolism of ruminants relative to monogastric animals, the physiological role of peripheral 5-HT on glucose and lipid metabolism in sheep remains to be established. Therefore, in this study, we investigated the effect of 5-HT on the circulating concentrations of metabolites and insulin using five 5-HT receptor (5HTR) antagonists in sheep. After fasting for 24 h, sheep were intravenously injected with 5-HT, following which-, plasma glucose, insulin, triglyceride and NEFA concentrations were significantly elevated. In contrast, 5-HT did not affect the plasma cholesterol concentration, and it induced a decrease in bile acid concentrations. Increases in plasma glucose and insulin concentrations induced by 5-HT were attenuated by pre-treatment with Methysergide, a 5HTR 1, 2 and 7 antagonist. Additionally, decreased plasma bile acid concentrations induced by 5-HT were blocked by pre-treatment with Ketanserin, a 5HTR 2A antagonist. However, none of the 5HTR antagonists inhibited the increase in plasma triglyceride and NEFA levels induced by 5-HT. On the other hand, mRNA expressions of 5HTR1D and 1E were observed in the liver, pancreas and skeletal muscle. These results suggest that there are a number of differences in the physiological functions of peripheral 5-HT with respect to lipid metabolism between mice and sheep, though its effect on glucose metabolism appears to be similar between these species.

Indexed as

AnimalsBile Acids and SaltsBlood GlucoseCholesterolFatty Acids, NonesterifiedGlucoseInsulinLipid MetabolismLiverMuscle, SkeletalPancreasReceptors, SerotoninSerotoninSerotonin AntagonistsSheepTriglyceridesBile Acids and SaltsBlood GlucoseCholesterolFatty Acids, NonesterifiedGlucoseInsulinReceptors, SerotoninSerotoninSerotonin AntagonistsTriglycerides

Identifiers

PMID24505376
PMCPMC3913723
OpenAlexW1966918560

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.