Evidence map›Paper›PMID 24847880›Full record

ArticleNature2014

Metformin suppresses gluconeogenesis by inhibiting mitochondrial glycerophosphate dehydrogenase.

Anila K Madiraju, Derek M Erion, Yasmeen Rahimi, Xian-Man Zhang, Demetrios T Braddock, Ronald A Albright, Brett J Prigaro, John L Wood, Sanjay Bhanot, Michael J MacDonald and 7 more

2 registry-linked trialsAbstract read
In one paragraph

Article in Nature, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to 2 registered trials, which are not on this map. Cited by 676 papers, 7 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
676citing papers in PubMed, 7 pooled it
51.2field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT03445702 early_phase1completedstarted 2018, after this paper: background citation

Metformin Gastrointestinal Intolerance: Measurement of Mitochondrial Complex I

Ran2018Enrolled15Registered outcomes2Posted comparisons0ConditionsDiabetes Mellitus, Type 2, Metformin Adverse ReactionArmsMetformin, Placebo
Open the trial in the graph
NCT05479214 phase4completedstarted 2022, after this paper: background citation

Effect of Adding Metformin to Insulin in Uncontrolled Diabetic Patients During the 3rd Trimester of Pregnancy on Glycemic Control, Fetal and Neonatal Outcomes ,Randomized Controlled Trial

Ran2022Enrolled150Registered outcomes8Posted comparisons0ConditionsDiabetes Mellitus PregnancyArmsInsulin, Metformin
Open the trial in the graph
3 · Its place in the literature

Who cites it

676 citing papers in PubMed, 7 syntheses or guidelines pooled it, 1,265 citations in OpenAlex.

  1. Pooled it
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  5. Influence of Solute Carrier Family 22 Member 1 (Current diabetes reviews · 2024
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616 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

17 authors at 7 institutions in 2 countries.

Anila K Madiraju1] Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520, USA [2] Department of Cellular & Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA [3] Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
Derek M Erion1] Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520, USA [2] Department of Cellular & Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA [3] Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
Yasmeen RahimiDepartment of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
Xian-Man ZhangDepartment of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
Demetrios T BraddockDepartment of Pathology, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
Ronald A AlbrightDepartment of Pathology, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
Brett J PrigaroDepartment of Chemistry, Colorado State University, Fort Collins, Colorado 80523, USA.
John L WoodCancer Prevention Research Institute of Texas Scholar, Department of Chemistry and Biochemistry, Baylor University, Waco, Texas 76798, USA.
Sanjay BhanotIsis Pharmaceuticals, 2855 Gazelle Court, Carlsbad, California 92010, USA.
Michael J MacDonaldUniversity of Wisconsin School of Medicine and Public Health, Madison, Wisconsin, USA, 53706.
Michael J JurczakDepartment of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
Joao-Paulo CamporezDepartment of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
Hui-Young LeeDepartment of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
Gary W ClineDepartment of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
Varman T SamuelDepartment of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
Richard G Kibbey1] Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520, USA [2] Department of Cellular & Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
Gerald I Shulman1] Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520, USA [2] Department of Cellular & Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA [3] Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut 06520, USA [4] Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Denmark, DK-2200.
Yale University · USBaylor University · USColorado State University · USHoward Hughes Medical Institute · USIonis Pharmaceuticals (United States) · USUniversity of Copenhagen · DKUniversity of Wisconsin–Madison · US

Funding

Yale Diabetes Research CenterP30DK045735 · NIDDK · YALE UNIVERSITY · PI GERALD I SHULMAN · 1993 to 2026
$44.0M
Yale University Clinical and Translational Science Award ProgramUL1TR000142 · NCATS · YALE UNIVERSITY · PI SHERWIN, ROBERT S · 2012 to 2015
$31.8M
Yale Liver CenterP30DK034989 · NIDDK · YALE UNIVERSITY · PI MICHAEL H NATHANSON, Mario Strazzabosco · 1986 to 2026
$31.1M
Yale Mouse Metabolic Phenotyping CenterU24DK059635 · NIDDK · YALE UNIVERSITY · PI SHULMAN, GERALD I · 2001 to 2015
$8.9M
GLUCOSE/SECRETAGOGUE METABOLISM IN PANCREATIC ISLETSR01DK028348 · NIDDK · UNIVERSITY OF WISCONSIN-MADISON · PI MACDONALD, MICHAEL JOHN · 1986 to 2013
$7.0M
Genetic and Cellular Mechanisms of NAFLD and Hepatic Insulin ResistanceR24DK085638 · NIDDK · YALE UNIVERSITY · PI SHULMAN, GERALD I · 2010 to 2014
$6.3M
Mechanisms of Lipid-Induced Hepatic Insulin ResistanceR01DK040936 · NIDDK · YALE UNIVERSITY · PI SHULMAN, GERALD I · 1989 to 2015
$5.1M
The role of the mitochondrial GTP cycle in insulin secretionR01DK092606 · NIDDK · YALE UNIVERSITY · PI KIBBEY, RICHARD G · 2011 to 2015
$1.8M
Parkin's role in hepatic mitochondrial turnover, steatosis and insulin resistanceK01DK099402 · NIDDK · YALE UNIVERSITY · PI JURCZAK, MICHAEL J. · 2013 to 2015
$441k
Howard Hughes Medical InstituteNCATS NIH HHS UL1 TR000142NIDDK NIH HHS K01 DK-099402NIDDK NIH HHS K01 DK099402NIDDK NIH HHS P30 DK-034989NIDDK NIH HHS P30 DK034989NIDDK NIH HHS P30 DK045735NIDDK NIH HHS P30 DK-45735NIDDK NIH HHS R01 DK028348NIDDK NIH HHS R01 DK040936NIDDK NIH HHS R01 DK-092606NIDDK NIH HHS R01 DK092606NIDDK NIH HHS R01 DK-28348NIDDK NIH HHS R01 DK-40936NIDDK NIH HHS R24 DK-085638NIDDK NIH HHS R24 DK085638NIDDK NIH HHS U24 DK-059635NIDDK NIH HHS U24 DK059635
6 · The paper itself

Abstract

Metformin is considered to be one of the most effective therapeutics for treating type 2 diabetes because it specifically reduces hepatic gluconeogenesis without increasing insulin secretion, inducing weight gain or posing a risk of hypoglycaemia. For over half a century, this agent has been prescribed to patients with type 2 diabetes worldwide, yet the underlying mechanism by which metformin inhibits hepatic gluconeogenesis remains unknown. Here we show that metformin non-competitively inhibits the redox shuttle enzyme mitochondrial glycerophosphate dehydrogenase, resulting in an altered hepatocellular redox state, reduced conversion of lactate and glycerol to glucose, and decreased hepatic gluconeogenesis. Acute and chronic low-dose metformin treatment effectively reduced endogenous glucose production, while increasing cytosolic redox and decreasing mitochondrial redox states. Antisense oligonucleotide knockdown of hepatic mitochondrial glycerophosphate dehydrogenase in rats resulted in a phenotype akin to chronic metformin treatment, and abrogated metformin-mediated increases in cytosolic redox state, decreases in plasma glucose concentrations, and inhibition of endogenous glucose production. These findings were replicated in whole-body mitochondrial glycerophosphate dehydrogenase knockout mice. These results have significant implications for understanding the mechanism of metformin's blood glucose lowering effects and provide a new therapeutic target for type 2 diabetes.

Indexed as

AnimalsBlood GlucoseCells, CulturedDiabetes Mellitus, Type 2GluconeogenesisGlycerolphosphate DehydrogenaseHumansHypoglycemic AgentsInsulinInsulin SecretionLactic AcidLiverMaleMetforminMice, KnockoutMitochondriaBlood GlucoseGlycerolphosphate DehydrogenaseHypoglycemic AgentsInsulinLactic AcidMetformin

Identifiers

PMID24847880
PMCPMC4074244
OpenAlexW2047516613

What Socratic holds

Texttitle and abstract
LicenceTDM
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.