ArticlePLoS genetics2014
N-wasp is required for structural integrity of the blood-testis barrier.
Article in PLoS genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- Actin Cytoskeleton Dysregulation Links Testicular and Sperm Dysfunction in Type 1 Diabetes.International journal of molecular sciences · 2026Article
- Inflammation-Driven Remodeling of the Blood-Testis Barrier: Roles of Junctional Complexes, Actin Dynamics, and Kinase Signaling.Biomedicines · 2026Review
- Conditional Knockout of N-WASP Enhanced the Formation of Keratinizing Squamous Cell Carcinoma Induced by KRasCancers · 2023Article
- mTORC1/rpS6 and mTORC2/PKC regulate spermatogenesis through Arp3-mediated actin microfilament organization in Eriocheir sinensis.Cell and tissue research · 2023Article
- FYN regulates cell adhesion at the blood-testis barrier and the apical ectoplasmic specializationFrontiers in immunology · 2022Article
- Dysfunction of dimorphic sperm impairs male fertility in the silkworm.Cell discovery · 2020Article
- Integrative Proteomic and Phosphoproteomic Profiling of Testis from Wip1 Phosphatase-Knockout Mice: Insights into Mechanisms of Reduced Fertility.Molecular & cellular proteomics : MCP · 2019Article
- Dynein 1 supports spermatid transport and spermiation during spermatogenesis in the rat testis.American journal of physiology. Endocrinology and metabolism · 2018Article
- Loss of N-WASP drives early progression in an Apc model of intestinal tumourigenesis.The Journal of pathology · 2018Article
- Monitoring the Integrity of the Blood-Testis Barrier (BTB): An In Vivo Assay.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Signaling pathways regulating blood-tissue barriers - Lesson from the testis.Biochimica et biophysica acta. Biomembranes · 2018Review
- Olaquindox disrupts tight junction integrity and cytoskeleton architecture in mouse Sertoli cells.Oncotarget · 2017Article
- SENP3 grants tight junction integrity and cytoskeleton architecture in mouse Sertoli cells.Oncotarget · 2017Article
- Regulation of spermatogenesis by a local functional axis in the testis: role of the basement membrane-derived noncollagenous 1 domain peptide.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2017Article
- Overexpression of plastin 3 in Sertoli cells disrupts actin microfilament bundle homeostasis and perturbs the tight junction barrier.Spermatogenesis · 2016Article
- Regulation of blood-testis barrier by actin binding proteins and protein kinases.Reproduction (Cambridge, England) · 2016Review
- AKAP9, a Regulator of Microtubule Dynamics, Contributes to Blood-Testis Barrier Function.The American journal of pathology · 2016Article
- Actin binding proteins in blood-testis barrier function.Current opinion in endocrinology, diabetes, and obesity · 2015Review
- Diet-induced obesity in male C57BL/6 mice decreases fertility as a consequence of disrupted blood-testis barrier.PloS one · 2015Article
- Formins: Actin nucleators that regulate cytoskeletal dynamics during spermatogenesis.SpermatogenesisArticle
Corrections and comments
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Authors and funding
12 authors at 5 institutions in 3 countries.
Funding
Abstract
During spermatogenesis, the blood-testis barrier (BTB) segregates the adluminal (apical) and basal compartments in the seminiferous epithelium, thereby creating a privileged adluminal environment that allows post-meiotic spermatid development to proceed without interference of the host immune system. A key feature of the BTB is its continuous remodeling within the Sertoli cells, the major somatic component of the seminiferous epithelium. This remodeling is necessary to allow the transport of germ cells towards the seminiferous tubule interior, while maintaining intact barrier properties. Here we demonstrate that the actin nucleation promoting factor Neuronal Wiskott-Aldrich Syndrome Protein (N-WASP) provides an essential function necessary for BTB restructuring, and for maintaining spermatogenesis. Our data suggests that the N-WASP-Arp2/3 actin polymerization machinery generates branched-actin arrays at an advanced stage of BTB remodeling. These arrays are proposed to mediate the restructuring process through endocytic recycling of BTB components. Disruption of N-WASP in Sertoli cells results in major structural abnormalities to the BTB, including mis-localization of critical junctional and cytoskeletal elements, and leads to disruption of barrier function. These impairments result in a complete arrest of spermatogenesis, underscoring the critical involvement of the somatic compartment of the seminiferous tubules in germ cell maturation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.