Evidence mapPaperPMID 25055153Full record

ArticlePloS one2014

A small amount of dietary carbohydrate can promote the HFD-induced insulin resistance to a maximal level.

Shuang Mei, Xuefeng Yang, Huailan Guo, Haihua Gu, Longying Zha, Junwei Cai, Xuefeng Li, Zhenqi Liu, Brian J Bennett, Ling He and 1 more

Open access · goldAbstract read
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed, 1 pooled it
0.3field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 1 synthesis or guideline pooled it, 12 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Review
  4. Article
  5. Article
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 2 countries.

Shuang MeiNutrition Research Institute at Kannapolis, Department of Nutrition, Gillings School of Global Public Health, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America.
Xuefeng YangDepartment of Nutrition, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America.
Huailan GuoDepartment of Nutrition, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America.
Haihua GuNutrition Research Institute at Kannapolis, Department of Nutrition, Gillings School of Global Public Health, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America.
Longying ZhaNutrition Research Institute at Kannapolis, Department of Nutrition, Gillings School of Global Public Health, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America; Department of Nutrition and Food Hygiene, South Medical University, Guangzhou, China.
Junwei CaiDepartment of Medicine, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
Xuefeng LiDepartment of Medicine, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
Zhenqi LiuDepartment of Medicine, University of Virginia Health System, Charlottesville, Virginia, United States of America.
Brian J BennettDepartment of Human Genetics, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America.
Ling HeDepartment of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America.
Wenhong CaoNutrition Research Institute at Kannapolis, Department of Nutrition, Gillings School of Global Public Health, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America; Department of Medicine, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China; Department of Medicine (Endocrinology and Metabolism), Duke University School of Medicine, Durham, North Carolina, United States of America.
University of North Carolina at Chapel Hill · USHubei University of Medicine · CNJohns Hopkins Medicine · USUniversity of Virginia Health System · US

Funding

NIDDK NIH HHS P30 DK079637NIDDK NIH HHS R00 DK085142NIDDK NIH HHS R01 DK076039NIDDK NIH HHS R01DK076039
6 · The paper itself

Abstract

Both dietary fat and carbohydrates (Carbs) may play important roles in the development of insulin resistance. The main goal of this study was to further define the roles for fat and dietary carbs in insulin resistance. C57BL/6 mice were fed normal chow diet (CD) or HFD containing 0.1-25.5% carbs for 5 weeks, followed by evaluations of calorie consumption, body weight and fat gains, insulin sensitivity, intratissue insulin signaling, ectopic fat, and oxidative stress in liver and skeletal muscle. The role of hepatic gluconeogenesis in the HFD-induced insulin resistance was determined in mice. The role of fat in insulin resistance was also examined in cultured cells. HFD with little carbs (0.1%) induced severe insulin resistance. Addition of 5% carbs to HFD dramatically elevated insulin resistance and 10% carbs in HFD was sufficient to induce a maximal level of insulin resistance. HFD with little carbs induced ectopic fat accumulation and oxidative stress in liver and skeletal muscle and addition of carbs to HFD dramatically enhanced ectopic fat and oxidative stress. HFD increased hepatic expression of key gluconeogenic genes and the increase was most dramatic by HFD with little carbs, and inhibition of hepatic gluconeogenesis prevented the HFD-induced insulin resistance. In cultured cells, development of insulin resistance induced by a pathological level of insulin was prevented in the absence of fat. Together, fat is essential for development of insulin resistance and dietary carb is not necessary for HFD-induced insulin resistance due to the presence of hepatic gluconeogenesis but a very small amount of it can promote HFD-induced insulin resistance to a maximal level.

Indexed as

Insulin ResistanceAnimalsCell LineCell Line, TumorCholesterolDietary CarbohydratesDiet, High-FatDose-Response Relationship, DrugFatty Acids, NonesterifiedFuransGluconeogenesisHypoglycemic AgentsImmunoblottingInsulinLiverMale5-(tetradecyloxy)-2-furancarboxylic acidCholesterolDietary CarbohydratesFatty Acids, NonesterifiedFuransHypoglycemic AgentsInsulinProto-Oncogene Proteins c-aktTriglycerides

Identifiers

PMID25055153
PMCPMC4108306
OpenAlexW2037573666

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.