Evidence map›Paper›PMID 25138607›Full record

Trial reportPhysiological genomics2014

Multi-omic integrated networks connect DNA methylation and miRNA with skeletal muscle plasticity to chronic exercise in Type 2 diabetic obesity.

David S Rowlands, Rachel A Page, William R Sukala, Mamta Giri, Svetlana D Ghimbovschi, Irum Hayat, Birinder S Cheema, Isabelle Lys, Murray Leikis, Phillip W Sheard and 12 more

Abstract readRandomized Controlled Trial
PubMed Full text at PMC Publisher
In one paragraph

Trial report in Physiological genomics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
70citing papers in PubMed, 3 pooled it
4.3field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

70 citing papers in PubMed, 3 syntheses or guidelines pooled it, 145 citations in OpenAlex.

  1. Pooled it
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  7. Effects of Exercise on ASC Methylation and IL-1 Cytokines in Heart Failure.Medicine and science in sports and exercise · 2018
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10 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors at 7 institutions in 3 countries.

David S RowlandsSchool of Sport and Exercise, Massey University, Wellington, New Zealand; D.S.Rowlands@massey.ac.nz.
Rachel A PageInstitute of Food, Nutrition & Human Health, Massey University, New Zealand;
William R SukalaInstitute of Food, Nutrition & Human Health, Massey University, New Zealand;
Mamta GiriChildren's National Medical Center, Center for Genetic Medicine Research (CGMR), Washington, District of Columbia;
Svetlana D GhimbovschiChildren's National Medical Center, Center for Genetic Medicine Research (CGMR), Washington, District of Columbia;
Irum HayatInstitute of Food, Nutrition & Human Health, Massey University, New Zealand;
Birinder S CheemaSchool of Science and Health, University of Western Sydney, Campbelltown, Australia;
Isabelle LysFaculty of Engineering, Health, Science and the Environment, Charles Darwin University, Australia;
Murray LeikisWellington Hospital, Capital and Coast District Health Board, Wellington, New Zealand;
Phillip W SheardDepartment of Physiology, University of Otago, Dunedin, New Zealand;
St John WakefieldDepartment of Pathology, University of Otago, Wellington, New Zealand; and.
Bernhard BreierInstitute of Food, Nutrition & Human Health, Massey University, New Zealand;
Yetrib HathoutChildren's National Medical Center, Center for Genetic Medicine Research (CGMR), Washington, District of Columbia;
Kristy BrownChildren's National Medical Center, Center for Genetic Medicine Research (CGMR), Washington, District of Columbia;
Ramya MarathiChildren's National Medical Center, Center for Genetic Medicine Research (CGMR), Washington, District of Columbia;
Funda E Orkunoglu-SuerChildren's National Medical Center, Center for Genetic Medicine Research (CGMR), Washington, District of Columbia;
Joseph M DevaneyChildren's National Medical Center, Center for Genetic Medicine Research (CGMR), Washington, District of Columbia;
Benjamin LeikenChildren's National Medical Center, Center for Genetic Medicine Research (CGMR), Washington, District of Columbia;
Gina ManyChildren's National Medical Center, Center for Genetic Medicine Research (CGMR), Washington, District of Columbia;
Jeremy KrebsEndocrine and Diabetes Unit, Capital and Coast District Health Board, Wellington, New Zealand;
Will G HopkinsHealth Science/Sport and Recreation, Auckland University of Technology, Auckland, New Zealand.
Eric P HoffmanChildren's National Medical Center, Center for Genetic Medicine Research (CGMR), Washington, District of Columbia;
Children's National · USMassey University · NZCapital and Coast District Health Board · NZUniversity of Otago · NZAuckland University of Technology · NZCharles Darwin University · AUWestern Sydney University · AU

Funding

Integrated Molecular Core for Rehabilitation MedicineR24HD050846 · CHILDREN'S RESEARCH INSTITUTE · 2005 to 2005
$706k
NICHD NIH HHS 2R24HD050846-06NICHD NIH HHS R24 HD050846
6 · The paper itself

Abstract

Epigenomic regulation of the transcriptome by DNA methylation and posttranscriptional gene silencing by miRNAs are potential environmental modulators of skeletal muscle plasticity to chronic exercise in healthy and diseased populations. We utilized transcriptome networks to connect exercise-induced differential methylation and miRNA with functional skeletal muscle plasticity. Biopsies of the vastus lateralis were collected from middle-aged Polynesian men and women with morbid obesity (44 kg/m(2) ± 10) and Type 2 diabetes before and following 16 wk of resistance (n = 9) or endurance training (n = 8). Longitudinal transcriptome, methylome, and microRNA (miRNA) responses were obtained via microarray, filtered by novel effect-size based false discovery rate probe selection preceding bioinformatic interrogation. Metabolic and microvascular transcriptome topology dominated the network landscape following endurance exercise. Lipid and glucose metabolism modules were connected to: microRNA (miR)-29a; promoter region hypomethylation of nuclear receptor factor (NRF1) and fatty acid transporter (SLC27A4), and hypermethylation of fatty acid synthase, and to exon hypomethylation of 6-phosphofructo-2-kinase and Ser/Thr protein kinase. Directional change in the endurance networks was validated by lower intramyocellular lipid, increased capillarity, GLUT4, hexokinase, and mitochondrial enzyme activity and proteome. Resistance training also lowered lipid and increased enzyme activity and caused GLUT4 promoter hypomethylation; however, training was inconsequential to GLUT4, capillarity, and metabolic transcriptome. miR-195 connected to negative regulation of vascular development. To conclude, integrated molecular network modelling revealed differential DNA methylation and miRNA expression changes occur in skeletal muscle in response to chronic exercise training that are most pronounced with endurance training and topographically associated with functional metabolic and microvascular plasticity relevant to diabetes rehabilitation.

Indexed as

ExerciseGene Regulatory NetworksDiabetes Mellitus, Type 2DNA MethylationEpigenesis, GeneticFemaleGene Expression RegulationGlucoseHumansLipid MetabolismMaleMicroRNAsMiddle AgedMuscle, SkeletalObesityPhenotypeGlucoseMicroRNAsRNA, Messengerdiabetes rehabilitationepigenomicintramyocellular lipidmyomiRsnetwork medicine

Identifiers

PMID25138607
PMCPMC4200377
OpenAlexW2121192580

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.