Evidence map›Paper›PMID 25144649›Full record

ArticlePloS one2014

Intralipid decreases apolipoprotein M levels and insulin sensitivity in rats.

Lu Zheng, Yuehua Feng, Yuanping Shi, Jun Zhang, Qinfeng Mu, Li Qin, Maria Berggren-Söderlund, Peter Nilsson-Ehle, Xiaoying Zhang, Guanghua Luo and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.9field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 18 citations in OpenAlex.

  1. Review
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  3. Sphingosine 1-Phosphate and Atherosclerosis.Journal of atherosclerosis and thrombosis · 2018
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 2 countries.

Lu ZhengComprehensive Laboratory, the Third Affiliated Hospital of Soochow University, Changzhou, P.R. China.
Yuehua FengComprehensive Laboratory, the Third Affiliated Hospital of Soochow University, Changzhou, P.R. China.
Yuanping ShiComprehensive Laboratory, the Third Affiliated Hospital of Soochow University, Changzhou, P.R. China.
Jun ZhangComprehensive Laboratory, the Third Affiliated Hospital of Soochow University, Changzhou, P.R. China.
Qinfeng MuComprehensive Laboratory, the Third Affiliated Hospital of Soochow University, Changzhou, P.R. China.
Li QinComprehensive Laboratory, the Third Affiliated Hospital of Soochow University, Changzhou, P.R. China.
Maria Berggren-SöderlundDivision of Clinical Chemistry and Pharmacology, Department of Laboratory Medicine, Lunds University, Lund, Sweden.
Peter Nilsson-EhleDivision of Clinical Chemistry and Pharmacology, Department of Laboratory Medicine, Lunds University, Lund, Sweden.
Xiaoying ZhangDepartment of Cardiothoracic Surgery, the Third Affiliated Hospital of Soochow University, Changzhou, P.R. China.
Guanghua LuoComprehensive Laboratory, the Third Affiliated Hospital of Soochow University, Changzhou, P.R. China.
Ning XuDivision of Clinical Chemistry and Pharmacology, Department of Laboratory Medicine, Lunds University, Lund, Sweden.
Soochow University · CNLund University · SE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundApolipoprotein M (ApoM) is a constituent of high-density lipoproteins (HDL). It plays a crucial role in HDL-mediated reverse cholesterol transport. Insulin resistance is associated with decreased ApoM levels.

aimsTo assess the effects of increased free fatty acids (FFAs) levels after short-term Intralipid infusion on insulin sensitivity and hepatic ApoM gene expression.

methodsAdult male Sprague-Dawley (SD) rats infused with 20% Intralipid solution for 6 h. Glucose infusion rates (GIR) were determined by hyperinsulinemic-euglycemic clamp during Intralipid infusion and plasma FFA levels were measured by colorimetry. Rats were sacrificed after Intralipid treatment and livers were sampled. Human embryonic kidney 293T cells were transfected with a lentivirus mediated human apoM overexpression system. Goto-Kakizaki (GK) rats were injected with the lentiviral vector and insulin tolerance was assessed. Gene expression was assessed by real-time RT-PCR and PCR array.

resultsIntralipid increased FFAs by 17.6 folds and GIR was decreased by 27.1% compared to the control group. ApoM gene expression was decreased by 40.4% after Intralipid infusion. PPARβ/δ expression was not changed by Intralipid. Whereas the mRNA levels of Acaca, Acox1, Akt1, V-raf murine sarcoma 3611 viral oncogene homolog, G6pc, Irs2, Ldlr, Map2k1, pyruvate kinase and RBC were significantly increased in rat liver after Intralipid infusion. The Mitogen-activated protein kinase 8 (MAPK8) was significantly down-regulated in 293T cells overexpressing ApoM. Overexpression of human ApoM in GK rats could enhance the glucose-lowering effect of exogenous insulin.

conclusionThese results suggest that Intralipid could decrease hepatic ApoM levels. ApoM overexpression may have a potential role in improving insulin resistance in vivo and modulating apoM expression might be a future therapeutic strategy against insulin resistance in type 2 diabetes.

Indexed as

Insulin ResistanceAnimalsApolipoproteinsApolipoproteins MFatty Acids, NonesterifiedGene ExpressionHEK293 CellsHumansInsulinLipocalinsLiverMalePPAR-betaPPAR deltaRatsRNA, MessengerApolipoproteinsApolipoproteins MAPOM protein, humanFatty Acids, NonesterifiedInsulinLipocalinsPPAR-betaPPAR deltaRNA, Messenger

Identifiers

PMID25144649
PMCPMC4140822
OpenAlexW2062778054

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.