ArticleAnalytica chimica acta2014
Imaging of epidermal growth factor receptor on single breast cancer cells using surface-enhanced Raman spectroscopy.
Article in Analytica chimica acta, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 29 citations in OpenAlex.
- Surface-Enhanced Raman Spectroscopy (SERS) Based Biological and Environmental 2D and 3D Imaging.ACS environmental Au · 2025Review
- Recent Trends in SERS-Based Plasmonic Sensors for Disease Diagnostics, Biomolecules Detection, and Machine Learning Techniques.Biosensors · 2023Review
- Comparison of SERS pH probe responses after microencapsulation within hydrogel matrices.Journal of biomedical optics · 2021Article
- Investigation of cellular uptake mechanism of functionalised gold nanoparticles into breast cancer using SERS.Chemical science · 2020Article
- Spatiotemporal dynamic monitoring of fatty acid-receptor interaction on single living cells by multiplexed Raman imaging.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- SERS-fluorescence bimodal nanoprobes for in vitro imaging of fatty acid responsive receptor GPR120.Analytical methods : advancing methods and applications · 2018Article
- Nanoparticles for live cell microscopy: A surface-enhanced Raman scattering perspective.Scientific reports · 2017Article
- GdSpectrochimica acta. Part A, Molecular and biomolecular spectroscopy · 2017Article
- Targeted-TERS detection of integrin receptors on human cancer cells.Cancer cell & microenvironment · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Epidermal growth factor receptor (EGFR) is widely used as a biomarker for pathological grading and therapeutic targeting of human cancers. This study investigates expression, spatial distribution as well as the endocytosis of EGFR in single breast cancer cells using surface-enhanced Raman spectroscopy (SERS). By incubating anti-EGFR antibody conjugated SERS nanoprobes with an EGFR-over-expressing cancer cell line, A431, EGFR localization was measured over time and found to be located primarily at the cell surface. To further validate the constructed SERS probes, we applied this SERS probes to detect the EGFR expression on breast cancer cells (MDA-MB-435, MDA-MB-231) and their counterpart cell lines in which EGFR expression was down-regulated by breast cancer metastasis suppressor 1 (BRMS1). The results showed that SERS method not only confirms immunoblot data measuring EGFR levels, but also adds new insights regarding EGFR localization and internalization in living cells which is impossible in immunoblot method. Thus, SERS provides a powerful new tool to measure biomarkers in living cancer cells.
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What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.