Evidence mapPaperPMID 25288790Full record

ArticleThe Journal of biological chemistry2014

Interaction between the pentose phosphate pathway and gluconeogenesis from glycerol in the liver.

Eunsook S Jin, A Dean Sherry, Craig R Malloy

2 registry-linked trialsOpen access · hybridAbstract read
In one paragraph

Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to 2 registered trials, which are not on this map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
1.3field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT02833415 phase4completedstarted 2016, after this paper: background citation

Visceral Adiposity and Diabetes: Translating Form to Function Using Imaging

Ran2016Enrolled40Registered outcomes1Posted comparisons2ConditionsObesity, VisceralArmsempagliflozin, Placebo (for Empagliflozin), [U-13C3] glycerol
Open the trial in the graph
NCT03480594 completednot on this mapstarted 2018, after this paper: background citation

Effect of Fatty Liver on TCA Cycle Flux and the Pentose Phosphate Pathway (HP FFF)

TypeobservationalSponsorUniversity of Texas Southwestern Medical CenterRan2018 to 2023Enrolled22ConditionsFatty LiverArmsMRI Tracer
3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 34 citations in OpenAlex.

  1. Review
  2. Article
  3. Magnetic Resonance Applications to Metabolism.Methods in molecular biology (Clifton, N.J.) · 2025
    Article
  4. Analysis ofFoods (Basel, Switzerland) · 2023
    Article
  5. Article
  6. Article
  7. Comparative transcriptomics reveal tissue level specialization towards diet in prickleback fishes.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2022
    Article
  8. Article
  9. Article
  10. Advances in stable isotope tracer methodology part 1: hepatic metabolism via isotopomer analysis and postprandial lipolysis modeling.Journal of investigative medicine : the official publication of the American Federation for Clinical Research · 2020
    Article
  11. Article
  12. Article
  13. Pentose phosphate pathway activity parallels lipogenesis but not antioxidant processes in rat liver.American journal of physiology. Endocrinology and metabolism · 2018
    Article
  14. Article
  15. Article
  16. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 3 institutions in 1 country.

Eunsook S JinFrom the Advanced Imaging Research Center and Departments of Internal Medicine and eunsook.jin@utsouthwestern.edu.
A Dean SherryFrom the Advanced Imaging Research Center and Radiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, the Department of Chemistry, University of Texas at Dallas, Richardson, Texas 75080, and.
Craig R MalloyFrom the Advanced Imaging Research Center and Departments of Internal Medicine and Radiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, the Veterans Affairs North Texas Health Care System, Dallas, Texas 75216.
Southwestern Medical Center · USThe University of Texas Southwestern Medical Center · USVA North Texas Health Care System · US

Funding

SPECIFIC GENE AND SMALL MOLECULE DELIVERY TO CELLSP01DK058398 · UNIVERSITY OF TEXAS SW MED CTR/DALLAS · 2000 to 2005
$6.9M
WORLD WIDE WEB HOME PAGE &CONSULTINGP41RR002584 · UNIVERSITY OF TEXAS SW MED CTR/DALLAS · 1985 to 2005
$6.2M
INTERMEDIARY METABOLISM IN THE HEART BY NMRR01HL034557 · UNIVERSITY OF TEXAS DALLAS · 1988 to 2005
$1.7M
NCRR NIH HHS P41 RR002584NCRR NIH HHS RR 002584NHLBI NIH HHS HL-34557NHLBI NIH HHS R01 HL034557NHLBI NIH HHS R37 HL034557NIBIB NIH HHS EB 015908NIBIB NIH HHS P41 EB015908NIDDK NIH HHS DK078933NIDDK NIH HHS DK099289NIDDK NIH HHS K01 DK078933NIDDK NIH HHS P01 DK058398NIDDK NIH HHS R01 DK099289
6 · The paper itself

Abstract

After exposure to [U-(13)C3]glycerol, the liver produces primarily [1,2,3-(13)C3]- and [4,5,6-(13)C3]glucose in equal proportions through gluconeogenesis from the level of trioses. Other (13)C-labeling patterns occur as a consequence of alternative pathways for glucose production. The pentose phosphate pathway (PPP), metabolism in the citric acid cycle, incomplete equilibration by triose phosphate isomerase, or the transaldolase reaction all interact to produce complex (13)C-labeling patterns in exported glucose. Here, we investigated (13)C labeling in plasma glucose in rats given [U-(13)C3]glycerol under various nutritional conditions. Blood was drawn at multiple time points to extract glucose for NMR analysis. Because the transaldolase reaction and incomplete equilibrium by triose phosphate isomerase cannot break a (13)C-(13)C bond within the trioses contributing to glucose, the appearance of [1,2-(13)C2]-, [2,3-(13)C2]-, [5,6-(13)C2]-, and [4,5-(13)C2]glucose provides direct evidence for metabolism of glycerol in the citric acid cycle or the PPP but not an influence of either triose phosphate isomerase or the transaldolase reaction. In all animals, [1,2-(13)C2]glucose/[2,3-(13)C2]glucose was significantly greater than [5,6-(13)C2]glucose/[4,5-(13)C2]glucose, a relationship that can only arise from gluconeogenesis followed by passage of substrates through the PPP. In summary, the hepatic PPP in vivo can be detected by (13)C distribution in blood glucose after [U-(13)C3]glycerol administration.

Indexed as

GluconeogenesisPentose Phosphate PathwayAnimalsBlood GlucoseCarbon IsotopesGlycerolLactatesLiverMagnetic Resonance SpectroscopyMaleRats, Sprague-DawleyTime FactorsTransaldolaseTriose-Phosphate IsomeraseBlood GlucoseCarbon IsotopesGlycerolLactatesTransaldolaseTriose-Phosphate IsomeraseGluconeogenesisGlucose MetabolismGlycerolLiver MetabolismNuclear Magnetic Resonance (NMR)Pentose Phosphate Pathway (PPP)

Identifiers

PMID25288790
PMCPMC4239613
OpenAlexW2067236516

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.