Evidence mapPaperPMID 25418322Full record

ArticleJournal of lipid research2015

In vivo 2H2O administration reveals impaired triglyceride storage in adipose tissue of insulin-resistant humans.

Candice A Allister, Li-fen Liu, Cindy A Lamendola, Colleen M Craig, Samuel W Cushman, Marc K Hellerstein, Tracey L McLaughlin

Open access · hybridAbstract read
In one paragraph

Article in Journal of lipid research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed, 1 pooled it
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.

  1. Pooled it
  2. Turnover Rate of Lipids, Metabolites and Proteins Revealed by 156-Day-Long DInternational journal of molecular sciences · 2026
    Article
  3. Article
  4. Review
  5. Review
  6. Deuterated water (Frontiers in immunology · 2025
    Article
  7. Article
  8. Review
  9. Adipose Tissue and Metabolic Health.Diabetes & metabolism journal · 2023
    Review
  10. Untargeted Lipidomics after DInternational journal of molecular sciences · 2023
    Article
  11. Review
  12. Article
  13. The acromegaly lipodystrophy.Frontiers in endocrinology · 2022
    Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Metabolically healthy obesity: facts and fantasies.The Journal of clinical investigation · 2019
    Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Candice A AllisterDepartment of Nutritional Science and Toxicology, University of California at Berkeley, Berkeley, CA.
Li-fen LiuDepartment of Medicine, Stanford University School of Medicine, Stanford, CA.
Cindy A LamendolaDepartment of Medicine, Stanford University School of Medicine, Stanford, CA.
Colleen M CraigDepartment of Medicine, Stanford University School of Medicine, Stanford, CA.
Samuel W CushmanDiabetes, Endocrinology, and Obesity Branch, National Institute of Digestive Diseases and Kidney Disease, National Institutes of Health, Bethesda, MD.
Marc K HellersteinDepartment of Nutritional Science and Toxicology, University of California at Berkeley, Berkeley, CA.
Tracey L McLaughlinDepartment of Medicine, Stanford University School of Medicine, Stanford, CA.
Stanford University · USUniversity of California, Berkeley · USNational Institutes of Health · US

Funding

UCSF Liver CenterP30DK026743 · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · 1986 to 2025
$6.5M
Integrating the Metabolic and Genetic Faces of ObesityR01DK071309 · STANFORD UNIVERSITY · 2005 to 2005
$345k
NCATS NIH HHS KL2 TR001083NIDDK NIH HHS P30 DK026743NIDDK NIH HHS R01 DK071309NIDDK NIH HHS R01DK071309NIDDK NIH HHS R01 DK080436
6 · The paper itself

Abstract

Indirect evidence suggests that impaired triglyceride storage in the subcutaneous fat depot contributes to the development of insulin resistance via lipotoxicity. We directly tested this hypothesis by measuring, in vivo, TG synthesis, de novo lipogenesis (DNL), adipocyte proliferation, and insulin suppression of lipolysis in subcutaneous adipose tissue of BMI-matched individuals classified as insulin resistant (IR) or insulin sensitive (IS). Nondiabetic, moderately obese subjects with BMI 25-35 kg/m(2), classified as IR or IS by the modified insulin suppression test, consumed deuterated water ((2)H2O) for 4 weeks. Deuterium incorporation into glycerol, palmitate, and DNA indicated TG synthesis, DNL, and adipocyte proliferation, respectively. Net TG synthesis and DNL in adipose cells were significantly lower in IR as compared with IS subjects, whereas adipocyte proliferation did not differ significantly. Plasma FFAs measured during an insulin suppression test were 2.5-fold higher in IR subjects, indicating resistance to insulin suppression of lipolysis. Adipose TG synthesis correlated directly with DNL but not with proliferation. These results provide direct in vivo evidence for impaired TG storage in subcutaneous adipose tissue of IR as compared with IS. Relative inability to store TG in the subcutaneous depot may represent a mechanism contributing to the development of insulin resistance in the setting of obesity.

Indexed as

Adipose TissueBody Mass IndexDeuterium OxideFemaleHumansInsulin ResistanceLipolysisMaleMiddle AgedObesityTriglyceridesDeuterium OxideTriglyceridesde novoinsulin resistancelipogenesislipolysisobesitysubcutaneous adiposetriglycerides

Identifiers

PMID25418322
PMCPMC4306696
OpenAlexW2167843550

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.