Evidence map›Paper›PMID 25447342›Full record

ArticleProstaglandins & other lipid mediators

Ethanol at low concentrations protects glomerular podocytes through alcohol dehydrogenase and 20-HETE.

Ellen T McCarthy, Jianping Zhou, Ryan Eckert, David Genochio, Rishi Sharma, Olurinde Oni, Alok De, Tarak Srivastava, Ram Sharma, Virginia J Savin and 1 more

Abstract read
In one paragraph

Article in Prostaglandins & other lipid mediators. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
1.7field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 25 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Observational
  5. Review
  6. Review
  7. Article
  8. Review
  9. Article
  10. Article
  11. Review
  12. Article
  13. Effect of Cytochrome P450 Metabolites of Arachidonic Acid in Nephrology.Journal of the American Society of Nephrology : JASN · 2017
    Review
  14. Impact of mild-to-moderate alcohol consumption and smoking on kidney function.Hypertension research : official journal of the Japanese Society of Hypertension · 2017
    Article
  15. The association of alcohol and smoking with CKD in a Japanese nationwide cross-sectional survey.Hypertension research : official journal of the Japanese Society of Hypertension · 2017
    Article
  16. Review
  17. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Ellen T McCarthyKidney Institute, University of Kansas Medical Center, Kansas City, KS, United States.
Jianping ZhouResearch Service, Kansas City VA Medical Center, Kansas City, MO, United States.
Ryan EckertResearch Service, Kansas City VA Medical Center, Kansas City, MO, United States.
David GenochioResearch Service, Kansas City VA Medical Center, Kansas City, MO, United States.
Rishi SharmaResearch Service, Kansas City VA Medical Center, Kansas City, MO, United States.
Olurinde OniResearch Service, Kansas City VA Medical Center, Kansas City, MO, United States.
Alok DeResearch Service, Kansas City VA Medical Center, Kansas City, MO, United States.
Tarak SrivastavaResearch Service, Kansas City VA Medical Center, Kansas City, MO, United States; Section of Nephrology, Children's Mercy Hospital and University of Missouri at Kansas City, Kansas City, MO, United States.
Ram SharmaResearch Service, Kansas City VA Medical Center, Kansas City, MO, United States.
Virginia J SavinKidney Institute, University of Kansas Medical Center, Kansas City, KS, United States; Research Service, Kansas City VA Medical Center, Kansas City, MO, United States.
Mukut SharmaKidney Institute, University of Kansas Medical Center, Kansas City, KS, United States; Research Service, Kansas City VA Medical Center, Kansas City, MO, United States. Electronic address: Mukut.sharma@va.gov.
Kansas City VA Medical Center · USUniversity of Kansas Medical Center · US

Funding

Novel Approaches for the Control of Microbial PathogensP30GM103326 · NIGMS · UNIVERSITY OF KANSAS MEDICAL CENTER · PI YANKEE, THOMAS M. · 2012 to 2016
$5.3M
CYTOCHROME P-450 AND GLOMERULAR PROTEIN PERMEABILITYR01DK064969 · NIDDK · UNIVERSITY OF KANSAS MEDICAL CENTER · PI MCCARTHY, ELLEN T · 2005 to 2010
$1.7M
Cytokine based murine focal glomerulosclerosis model a prelude to novel therapyI01BX001037 · VA · KANSAS CITY VA MEDICAL CENTER · PI SAVIN, VIRGINIA J. · 2011 to 2014
–
BLRD VA I01 BX001037NIDDK NIH HHS DK 1RO1DK064969NIDDK NIH HHS R01 DK064969NIGMS NIH HHS P30 GM103326
6 · The paper itself

Abstract

Clinical studies suggest cardiovascular and renal benefits of ingesting small amounts of ethanol. Effects of ethanol, role of alcohol dehydrogenase (ADH) or of 20-hydroxyeicosatetraenoic acid (20-HETE) in podocytes of the glomerular filtration barrier have not been reported. We found that mouse podocytes at baseline generate 20-HETE and express ADH but not CYP2e1. Ethanol at high concentrations altered the actin cytoskeleton, induced CYP2e1, increased superoxide production and inhibited ADH gene expression. Ethanol at low concentrations upregulated the expression of ADH and CYP4a12a. 20-HETE, an arachidonic acid metabolite generated by CYP4a12a, blocked the ethanol-induced cytoskeletal derangement and superoxide generation. Ethanol at high concentration or ADH inhibitor increased glomerular albumin permeability in vitro. 20-HETE and its metabolite produced by ADH activity, 20-carboxy-arachidonic acid, protected the glomerular permeability barrier against an ADH inhibitor, puromycin or FSGS permeability factor. We conclude that ADH activity is required for glomerular function, 20-HETE is a physiological substrate of ADH in podocytes and that podocytes are useful biosensors to understand glomeruloprotective effects of ethanol.

Indexed as

Alcohol DehydrogenaseAnimalsCell Line, TransformedCytochrome P-450 CYP2E1Cytochrome P-450 Enzyme SystemCytochrome P450 Family 4EthanolHydroxyeicosatetraenoic AcidsKidney DiseasesMicePodocytesProtein Synthesis InhibitorsPuromycin20-hydroxy-5,8,11,14-eicosatetraenoic acidAlcohol DehydrogenaseCyp4a12a protein, mouseCytochrome P-450 CYP2E1Cytochrome P-450 Enzyme SystemCytochrome P450 Family 4EthanolHydroxyeicosatetraenoic AcidsProtein Synthesis InhibitorsPuromycin20-Carboxy-arachidonic acid20-Hydroxyeicosatetraenoic AcidAlcohol dehydrogenaseChronic kidney diseaseEthanolGlomerular filtration barrierOxidative stressPodocytesProteinuria

Identifiers

PMID25447342
PMCPMC4385495
OpenAlexW2053614212

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.