ArticleMolecular biology of the cell2015
Characterization of VAMP isoforms in 3T3-L1 adipocytes: implications for GLUT4 trafficking.
Article in Molecular biology of the cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- Knockout of syntaxin-4 in 3T3-L1 adipocytes reveals new insight into GLUT4 trafficking and adiponectin secretion.Journal of cell science · 2022Article
- EFR3 and phosphatidylinositol 4-kinase IIIα regulate insulin-stimulated glucose transport and GLUT4 dispersal in 3T3-L1 adipocytes.Bioscience reports · 2022Article
- Heat Shock Alters the Proteomic Profile of Equine Mesenchymal Stem Cells.International journal of molecular sciences · 2022Article
- Pleiotropic effects of Syntaxin16 identified by gene editing in cultured adipocytes.Frontiers in cell and developmental biology · 2022Article
- Hepatocyte TMEM16A Deletion Retards NAFLD Progression by Ameliorating Hepatic Glucose Metabolic Disorder.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- Article
- Dysregulated autophagy in muscle precursor cells from humans with type 2 diabetes.Scientific reports · 2019Article
- Cardiac SNARE Expression in Health and Disease.Frontiers in endocrinology · 2019Article
- GLUT4 expression and glucose transport in human induced pluripotent stem cell-derived cardiomyocytes.PloS one · 2019Article
- VAMP2 is implicated in the secretion of antibodies by human plasma cells and can be replaced by other synaptobrevins.Cellular & molecular immunology · 2018Article
- The Motor KIF5C Links the Requirements of Stable Microtubules and IGF-1 Receptor Membrane Insertion for Neuronal Polarization.Molecular neurobiology · 2017Article
- VAMP3/Syb and YKT6 are required for the fusion of constitutive secretory carriers with the plasma membrane.PLoS genetics · 2017Article
- Calreticulin: Challenges Posed by the Intrinsically Disordered Nature of Calreticulin to the Study of Its Function.Frontiers in cell and developmental biology · 2017Article
- SNAP23/25 and VAMP2 mediate exocytic event of transferrin receptor-containing recycling vesicles.Biology open · 2015Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The fusion of GLUT4-containing vesicles with the plasma membrane of adipocytes is a key facet of insulin action. This process is mediated by the formation of functional soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes between the plasma membrane t-SNARE complex and the vesicle v-SNARE or VAMP. The t-SNARE complex consists of Syntaxin4 and SNAP23, and whereas many studies identify VAMP2 as the v-SNARE, others suggest that either VAMP3 or VAMP8 may also fulfil this role. Here we characterized the levels of expression, distribution, and association of all the VAMPs expressed in 3T3-L1 adipocytes to provide the first systematic analysis of all members of this protein family for any cell type. Despite our finding that all VAMP isoforms form SDS-resistant SNARE complexes with Syntaxin4/SNAP23 in vitro, a combination of levels of expression (which vary by >30-fold), subcellular distribution, and coimmunoprecipitation analyses lead us to propose that VAMP2 is the major v-SNARE involved in GLUT4 trafficking to the surface of 3T3-L1 adipocytes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.