ArticleThe Biochemical journal2015
Unravelling the pivotal role of Alix in MVB sorting and silencing of the activated EGFR.
Article in The Biochemical journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 34 citations in OpenAlex.
- Silencing the Signal: The Metastasis Suppressor NDRG1 Disrupts Small Extracellular Vesicle-Mediated Crosstalk in Pancreatic Cancer.Journal of extracellular vesicles · 2026Article
- Proteomic analysis of serum small extracellular vesicles identifies diagnostic biomarkers for neuroblastoma.Frontiers in oncology · 2024Article
- Indacaterol inhibits collective cell migration and IGDQ-mediated single cell migration in metastatic breast cancer MDA-MB-231 cells.Cell communication and signaling : CCS · 2023Article
- A new paradigm for epidermal growth factor receptor expression exists in PTC and NIFTP regulated by microRNAs.Frontiers in oncology · 2023Article
- Small extracellular vesicle PD-L1 in cancer: the knowns and unknowns.NPJ precision oncology · 2022Review
- Therapeutically harnessing extracellular vesicles.Nature reviews. Drug discovery · 2022Review
- Interactions of ubiquitin and CHMP5 with the V domain of HD-PTP reveals role for regulation of Vps4 ATPase.Molecular biology of the cell · 2021Article
- His domain protein tyrosine phosphatase and Rabaptin-5 couple endo-lysosomal sorting of EGFR with endosomal maturation.Journal of cell science · 2021Article
- Bardet-Biedl syndrome proteins modulate the release of bioactive extracellular vesicles.Nature communications · 2021Article
- Cooperation and Interplay between EGFR Signalling and Extracellular Vesicle Biogenesis in Cancer.Cells · 2020Review
- Focus on the morphogenesis, fate and the role in tumor progression of multivesicular bodies.Cell communication and signaling : CCS · 2020Review
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- Alpha-synuclein is strategically positioned for afferent modulation of midbrain dopamine neurons and is essential for cocaine preference.Communications biology · 2019Article
- Skeletal Muscle-Released Extracellular Vesicles: State of the Art.Frontiers in physiology · 2019Review
- Dissecting the role of His domain protein tyrosine phosphatase/PTPN23 and ESCRTs in sorting activated epidermal growth factor receptor to the multivesicular body.Biochemical Society transactions · 2018Review
- The open architecture of HD-PTP phosphatase provides new insights into the mechanism of regulation of ESCRT function.Scientific reports · 2017Article
- Tumor susceptibility gene 101 regulates predisposition to apoptosis via ESCRT machinery accessory proteins.Molecular biology of the cell · 2017Article
- Structural Basis for Selective Interaction between the ESCRT Regulator HD-PTP and UBAP1.Structure (London, England : 1993) · 2016Article
- The role of CHMP2BBrain research · 2016Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Endosomal sorting complex required for transport (ESCRT)-III-mediated membrane invagination and scission are a critical step in multivesicular body (MVB) sorting of ubiquitinated membrane receptors, and generally thought to be required for degradation of these receptors in lysosomes. The adaptor protein Alix is critically involved in multiple ESCRT-III-mediated, membrane-remodelling processes in mammalian cells. However, Alix knockdown does not inhibit degradation of the activated epidermal growth factor receptor (EGFR) in mammalian cell lines, leading to a widely held notion that Alix is not critically involved in MVB sorting of ubiquitinated membrane receptors in mammalian cells. In the present study, we demonstrate that, despite its non-essential role in degradation of the activated EGFR, Alix plays a critical role in its MVB sorting and silencing Epidermal growth factor (EGF) stimulation of mammalian cell lines induces Alix's interaction with the ubiquitinated EGFR via the Alix V domain, and increases Alix's association with membrane-bound charged multivesicular body protein 4 (CHMP4) via the Alix Bro1 domain. Under both continuous and pulse-chase EGF stimulation conditions, inhibition of Alix's interaction with membrane-bound CHMP4, inhibition of Alix dimerization through the V domain or Alix knockdown dramatically inhibits MVB sorting of the activated EGFR and promotes sustained activation of extracellular-signal regulated kinase (ERK)1/2. Under the continuous EGF stimulation conditions, these cell treatments also retard degradation of the activated EGFR. These findings indicate that Alix is critically involved in MVB sorting of ubiquitinated membrane receptors in mammalian cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.