ArticleBMC biology2014
The Lingulodinium circadian system lacks rhythmic changes in transcript abundance.
Article in BMC biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 47 citations in OpenAlex.
- Circadian rhythms: pervasive, and often times evasive.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- Abundant mRNA mEMBO reports · 2024Article
- Genome-wide transcriptome analysis reveals the diversity and function of long non-coding RNAs in dinoflagellates.NAR genomics and bioinformatics · 2024Article
- The Case for the Target of Rapamycin Pathway as a Candidate Circadian Oscillator.International journal of molecular sciences · 2023Review
- Insights into daily metabolic changes of the dinoflagellateCell cycle (Georgetown, Tex.) · 2023Review
- Orchestrated translation specializes dinoflagellate metabolism three times per day.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- The enigmatic clock of dinoflagellates, is it unique?Frontiers in microbiology · 2022Review
- De novo transcriptome assembly data of the marine bioluminescent dinoflagellateData in brief · 2021Article
- Biosynthesis of Saxitoxin in Marine Dinoflagellates: An Omics Perspective.Marine drugs · 2020Review
- Omics Analysis for Dinoflagellates Biology Research.Microorganisms · 2019Review
- Assessing Transcriptional Responses to Light by the DinoflagellateMicroorganisms · 2019Article
- Therapeutic Potential and Biological Applications of Cordycepin and Metabolic Mechanisms in Cordycepin-Producing Fungi.Molecules (Basel, Switzerland) · 2019Review
- Circadian rhythms, metabolic oscillators, and the target of rapamycin (TOR) pathway: the Neurospora connection.Current genetics · 2019Review
- The Plant Circadian Oscillator.Biology · 2019Review
- A period without PER: understanding 24-hour rhythms without classic transcription and translation feedback loops.F1000Research · 2019Review
- Non-transcriptional processes in circadian rhythm generation.Current opinion in physiology · 2018Review
- Translation and Translational Control in Dinoflagellates.Microorganisms · 2018Review
- A proteomic portrait of dinoflagellate chromatin reveals abundant RNA-binding proteins.Chromosoma · 2018Article
- What makes ribosomes tick?RNA biology · 2018Review
- Rhythmic potassium transport regulates the circadian clock in human red blood cells.Nature communications · 2017Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAlmost all cells display circadian rhythms, approximately 24-hour period changes in their biochemistry, physiology or behavior. These rhythms are orchestrated by an endogenous circadian clock whose mechanism is based on transcription-translation feedback loops (TTFL) where the translated products of clock genes act to inhibit their own transcription.
resultsWe have used RNA-Seq to measure the abundance of all transcripts in an RNA-Seq-derived de novo gene catalog in two different experiments. One compared midday and midnight in a light-dark cycle (ZT6 and ZT18) and under constant light (CT6 and CT18). The second compared four different times (ZT2, ZT6, ZT14 and ZT18) under a light dark cycle. We show here that despite an elaborate repertoire of biological rhythms, the unicellular dinoflagellate Lingulodinium had no detectable daily variation in the abundance of any transcript in an RNA-Seq-derived de novo gene catalog. We also examined the timing of the bioluminescence and photosynthesis rhythms in the presence of the transcription inhibitors actinomycin D and cordycepin. We found that the timing of the two rhythms was unchanged even when transcription rates had decreased to roughly 5% the levels of untreated cells.
conclusionsThe lack of detectable daily variation in transcript levels indicates that the endogenous circadian timer of Lingulodinium does not require rhythmic RNA. If the circadian timer is considered as a limit cycle oscillator, then cellular time in this organism must be defined by variations in state variables that do not include the amount of a clock gene transcript.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.