ArticleHaematologica2015
Dexamethasone targeted directly to macrophages induces macrophage niches that promote erythroid expansion.
Article in Haematologica, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 52 citations in OpenAlex.
- Impaired formation of erythroblastic islands is associated with erythroid failure and poor prognosis in a significant proportion of patients with myelodysplastic syndromes.Haematologica · 2016Trial
- CD163Biomarker research · 2026Article
- Resolution of inflammation increases erythropoiesis.Blood red cells & iron · 2025Article
- Article
- Immunoregulation role of the erythroid cells.Frontiers in immunology · 2024Review
- CD169-CD43 interaction is involved in erythroblastic island formation and erythroid differentiation.Haematologica · 2023Article
- Patients with hypercortisolemic Cushing disease possess a distinct class of hematopoietic progenitor cells leading to erythrocytosis.Haematologica · 2023Article
- Article
- Functions of CD169 positive macrophages in human diseases (Review).Biomedical reports · 2021Review
- Evolution and new frontiers of histology in bio-medical research.Microscopy research and technique · 2021Review
- CD14+ monocytes repress gamma globin expression at early stages of erythropoiesis.Scientific reports · 2021Article
- Transcriptional Control of Gene Expression and the Heterogeneous Cellular Identity of Erythroblastic Island Macrophages.Frontiers in genetics · 2021Review
- The Glucocorticoid Receptor Polymorphism Landscape in Patients With Diamond Blackfan Anemia Reveals an Association Between Two Clinically Relevant Single Nucleotide Polymorphisms and Time to Diagnosis.Frontiers in physiology · 2021Article
- Stress erythropoiesis: definitions and models for its study.Experimental hematology · 2020Review
- An Overview of Different Strategies to Recreate the Physiological Environment in Experimental Erythropoiesis.International journal of molecular sciences · 2020Review
- Ephrin/Eph receptor interaction facilitates macrophage recognition of differentiating human erythroblasts.Haematologica · 2020Article
- Review
- Large-scale in vitro production of red blood cells from human peripheral blood mononuclear cells.Blood advances · 2019Article
- Dexamethasone Predisposes Human Erythroblasts Toward Impaired Lipid Metabolism and Renders TheirFrontiers in physiology · 2019Article
- Phosphoproteomic Landscaping Identifies Non-canonical cKIT Signaling in Polycythemia Vera Erythroid Progenitors.Frontiers in oncology · 2019Article
Corrections and comments
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Authors and funding
12 authors at 6 institutions in 3 countries.
Funding
Abstract
Cultures of human CD34(pos) cells stimulated with erythroid growth factors plus dexamethasone, a model for stress erythropoiesis, generate numerous erythroid cells plus a few macrophages (approx. 3%; 3:1 positive and negative for CD169). Interactions occurring between erythroblasts and macrophages in these cultures and the biological effects associated with these interactions were documented by live phase-contrast videomicroscopy. Macrophages expressed high motility interacting with hundreds/thousands of erythroblasts per hour. CD169(pos) macrophages established multiple rapid 'loose' interactions with proerythroblasts leading to formation of transient erythroblastic island-like structures. By contrast, CD169(neg) macrophages established 'tight' interactions with mature erythroblasts and phagocytosed these cells. 'Loose' interactions of CD169(pos) macrophages were associated with proerythroblast cytokinesis (the M phase of the cell cycle) suggesting that these interactions may promote proerythroblast duplication. This hypothesis was tested by experiments that showed that as few as 103 macrophages significantly increased levels of 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide incorporation frequency in S/G2/M and cytokinesis expressed by proerythroblasts over 24 h of culture. These effects were observed also when macrophages were co-cultured with dexamethasone directly conjugated to a macrophage-specific CD163 antibody. In conclusion, in addition to promoting proerythroblast proliferation directly, dexamethasone stimulates expansion of these cells indirectly by stimulating maturation and cytokinesis supporting activity of macrophages.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.