ArticlePLoS genetics2015
Deciphering the genetic programme triggering timely and spatially-regulated chitin deposition.
Article in PLoS genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 58 citations in OpenAlex.
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- Distinct cellular and molecular mechanisms contribute to the specificity of the two Drosophila melanogaster chitin synthases in chitin deposition.PLoS genetics · 2025Article
- Cell-Mediated Branch Fusion in the Drosophila Trachea.Results and problems in cell differentiation · 2024Review
- The Osiris family genes function as novel regulators of the tube maturation process in the Drosophila trachea.PLoS genetics · 2023Article
- Host-induced silencing of the CpCHI gene resulted in developmental abnormalities and mortality in maize stem borer (Chilo partellus).PloS one · 2023Article
- A dynamic interplay between chitin synthase and the proteins Expansion/Rebuf reveals that chitin polymerisation and translocation are uncoupled in Drosophila.PLoS biology · 2023Article
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- Molecular Characterization of UDP-Genes · 2022Article
- Mitochondria as environments for the nuclear genome in Drosophila: mitonuclear G×G×E.The Journal of heredity · 2022Article
- Glycosylhydrolase genes control respiratory tubes sizes and airway stability.Scientific reports · 2020Article
- Extracellular matrix dynamics in tubulogenesis.Cellular signalling · 2020Review
- Form and function of the apical extracellular matrix: new insights fromFaculty reviews · 2020Review
- Molecular cloning, expression, and functional analysis of the chitin synthase 1 gene and its two alternative splicing variants in the white-backed planthopper, Sogatella furcifera (Hemiptera: Delphacidae).Scientific reports · 2019Article
- Blimp-1 Mediates Tracheal Lumen Maturation inGenetics · 2018Article
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- An ancient family of lytic polysaccharide monooxygenases with roles in arthropod development and biomass digestion.Nature communications · 2018Article
- An Ichor-dependent apical extracellular matrix regulates seamless tube shape and integrity.PLoS genetics · 2018Article
- Time to make the doughnuts: Building and shaping seamless tubes.Seminars in cell & developmental biology · 2017Review
- Chitinases and Imaginal disc growth factors organize the extracellular matrix formation at barrier tissues in insects.Scientific reports · 2016Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Organ and tissue formation requires a finely tuned temporal and spatial regulation of differentiation programmes. This is necessary to balance sufficient plasticity to undergo morphogenesis with the acquisition of the mature traits needed for physiological activity. Here we addressed this issue by analysing the deposition of the chitinous extracellular matrix of Drosophila, an essential element of the cuticle (skin) and respiratory system (tracheae) in this insect. Chitin deposition requires the activity of the chitin synthase Krotzkopf verkehrt (Kkv). Our data demonstrate that this process equally requires the activity of two other genes, namely expansion (exp) and rebuf (reb). We found that Exp and Reb have interchangeable functions, and in their absence no chitin is produced, in spite of the presence of Kkv. Conversely, when Kkv and Exp/Reb are co-expressed in the ectoderm, they promote chitin deposition, even in tissues normally devoid of this polysaccharide. Therefore, our results indicate that both functions are not only required but also sufficient to trigger chitin accumulation. We show that this mechanism is highly regulated in time and space, ensuring chitin accumulation in the correct tissues and developmental stages. Accordingly, we observed that unregulated chitin deposition disturbs morphogenesis, thus highlighting the need for tight regulation of this process. In summary, here we identify the genetic programme that triggers the timely and spatially regulated deposition of chitin and thus provide new insights into the extracellular matrix maturation required for physiological activity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.