Evidence map›Paper›PMID 25629157›Full record

ArticlePloS one2015

Rapid screening of gene function by systemic delivery of morpholino oligonucleotides to live mouse embryos.

Kathryn S McClelland, Elanor N Wainwright, Josephine Bowles, Peter Koopman

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 16 citations in OpenAlex.

  1. Article
  2. ADAMTS-3, -13, -16, and -19 levels in patients with habitual abortion.The Kaohsiung journal of medical sciences · 2017
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Kathryn S McClellandInstitute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland, Australia.
Elanor N WainwrightInstitute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland, Australia.
Josephine BowlesInstitute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland, Australia.
Peter KoopmanInstitute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland, Australia.
University of Queensland · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Traditional gene targeting methods in mice are complex and time consuming, especially when conditional deletion methods are required. Here, we describe a novel technique for assessing gene function by injection of modified antisense morpholino oligonucleotides (MOs) into the heart of mid-gestation mouse embryos. After allowing MOs to circulate through the embryonic vasculature, target tissues were explanted, cultured and analysed for expression of key markers. We established proof-of-principle by partially phenocopying known gene knockout phenotypes in the fetal gonads (Stra8, Sox9) and pancreas (Sox9). We also generated a novel double knockdown of Gli1 and Gli2, revealing defects in Leydig cell differentiation in the fetal testis. Finally, we gained insight into the roles of Adamts19 and Ctrb1, genes of unknown function in sex determination and gonadal development. These studies reveal the utility of this method as a means of first-pass analysis of gene function during organogenesis before committing to detailed genetic analysis.

Indexed as

ADAM ProteinsAdaptor Proteins, Signal TransducingAnimalsChymotrypsinEmbryo, MammalianEmbryonic DevelopmentFemaleGene Knockout TechniquesGene TargetingGonadsInjectionsKruppel-Like Transcription FactorsMaleMiceMorpholinosOrganogenesisADAM ProteinsAdaptor Proteins, Signal TransducingChymotrypsinchymotrypsin BGli1 protein, mouseKruppel-Like Transcription FactorsMorpholinosSOX9 Transcription FactorStra8 protein, mouseZinc Finger Protein GLI1

Identifiers

PMID25629157
PMCPMC4309589
OpenAlexW2053224627

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.