ArticleThe Plant cell2015
Predictable alteration of sequence recognition by RNA editing factors from Arabidopsis.
Article in The Plant cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
46 citing papers in PubMed.
- Expanding the palette of cis-elements to tune the expression of chloroplast transgenes in tobacco.The Plant journal : for cell and molecular biology · 2026Article
- Natural Variation of a PPR Coding Gene SST1 Confers Salt Tolerance During Soybean Domestication.Plant biotechnology journal · 2026Article
- Synthetic Pentatricopeptide Repeat Proteins: Building a Toolkit for Precise RNA Control.International journal of molecular sciences · 2025Review
- GRASP: a modular toolkit for building synthetic pentatricopeptide repeat RNA-binding proteins.Nucleic acids research · 2025Article
- Evolution of mitochondrial RNA editing sites and stop codon-lacking transcripts in angiosperms.Communications biology · 2025Article
- De novo RNA base editing in plant organelles with engineered synthetic P-type PPR editing factors.Nucleic acids research · 2025Article
- Applications of Synthetic Pentatricopeptide Repeat Proteins.Plant & cell physiology · 2024Review
- Alteration of Mitochondrial Transcript Expression inInternational journal of molecular sciences · 2023Article
- Interplay of endonucleolytic and exonucleolytic processing in the 3'-end formation of a mitochondrial nad2 RNA precursor in Arabidopsis.Nucleic acids research · 2023Article
- Plant mitochondrial RNA editing factors can perform targeted C-to-U editing of nuclear transcripts in human cells.Nucleic acids research · 2022Article
- U-to-C RNA editing by synthetic PPR-DYW proteins in bacteria and human culture cells.Communications biology · 2022Article
- A synthetic RNA editing factor edits its target site in chloroplasts and bacteria.Communications biology · 2021Article
- The genetic basis of cytoplasmic male sterility and fertility restoration in wheat.Nature communications · 2021Article
- Cofactor-independent RNA editing by a synthetic S-type PPR protein.Synthetic biology (Oxford, England) · 2021Article
- The PPR-SMR Protein ATP4 Is Required for Editing the ChloroplastPlant physiology · 2020Article
- One C-to-U RNA Editing Site and Two Independently Evolved Editing Factors: Testing Reciprocal Complementation with DYW-Type PPR Proteins from the MossThe Plant cell · 2020Article
- Single and Combined Methods to Specifically or Bulk-Purify RNA-Protein Complexes.Biomolecules · 2020Review
- The OPR Protein MTHI1 Controls the Expression of Two Different Subunits of ATP Synthase CFo inThe Plant cell · 2020Article
- The Analysis of the Editing Defects in thePlants (Basel, Switzerland) · 2020Article
- Pentatricopeptide repeat protein MID1 modulates nad2 intron 1 splicing and Arabidopsis development.Scientific reports · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA editing factors of the pentatricopeptide repeat (PPR) family show a very high degree of sequence specificity in the recognition of their target sites. A molecular basis for target recognition by editing factors has been proposed based on statistical correlations but has not been tested experimentally. To achieve this, we systematically mutated the pentatricopeptide motifs in the Arabidopsis thaliana RNA editing factor CLB19 to investigate their individual contribution to RNA recognition. We find that the motifs contributing significantly to the specificity of binding follow the previously proposed recognition rules, distinguishing primarily between purines and pyrimidines. Our results are consistent with proposals that each motif recognizes one nucleotide in the RNA target with the protein aligned parallel to the RNA and contiguous motifs aligned with contiguous nucleotides such that the final PPR motif aligns four nucleotides upstream of the edited cytidine. By altering S motifs in CLB19 and another editing factor, OTP82, and using the modified proteins to attempt to complement the respective mutants, we demonstrate that we can predictably alter the specificity of these factors in vivo.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.