ArticleNucleic acids research2015
bHLH proteins involved in Drosophila neurogenesis are mutually regulated at the level of stability.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Fluorescent labeling of proteins in vitro and in vivo using encoded peptide tags.The Journal of biological chemistry · 2025Review
- The Id protein Extramacrochaetae restrains the E protein Daughterless to regulate Notch, Rap1, and Sevenless within the R7 equivalence group of the Drosophila eye.Biology open · 2024Article
- The herpesvirus UL49.5 protein hijacks a cellular C-degron pathway to drive TAP transporter degradation.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Article
- New Insights Into the Intricacies of Proneural Gene Regulation in the Embryonic and Adult Cerebral Cortex.Frontiers in molecular neuroscience · 2021Review
- Evolutionary Variation in MADS Box Dimerization Affects Floral Development and Protein Abundance in Maize.The Plant cell · 2020Article
- Regulation of Notch output dynamics via specific E(spl)-HLH factors during bristle patterning in Drosophila.Nature communications · 2019Article
- Multi-site phosphorylation controls the neurogenic and myogenic activity of E47.Biochemical and biophysical research communications · 2019Article
- N-terminal phosphorylation of xHes1 controls inhibition of primary neurogenesis in Xenopus.Biochemical and biophysical research communications · 2019Article
- All in the family: proneural bHLH genes and neuronal diversity.Development (Cambridge, England) · 2018Review
- Regulation of theeLife · 2018Article
- Subcellular localisation modulates ubiquitylation and degradation of Ascl1.Scientific reports · 2018Article
- The dynamics of native Atoh7 protein expression during mouse retinal histogenesis, revealed with a new antibody.Gene expression patterns : GEP · 2018Article
- Article
- E Proteins and ID Proteins: Helix-Loop-Helix Partners in Development and Disease.Developmental cell · 2015Review
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3 authors.
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Abstract
Proneural bHLH activators are expressed in all neuroectodermal regions prefiguring events of central and peripheral neurogenesis. Drosophila Sc is a prototypical proneural activator that heterodimerizes with the E-protein Daughterless (Da) and is antagonized by, among others, the E(spl) repressors. We determined parameters that regulate Sc stability in Drosophila S2 cells. We found that Sc is a very labile phosphoprotein and its turnover takes place via at least three proteasome-dependent mechanisms. (i) When Sc is in excess of Da, its degradation is promoted via its transactivation domain (TAD). (ii) In a DNA-bound Da/Sc heterodimer, Sc degradation is promoted via an SPTSS phosphorylation motif and the AD1 TAD of Da; Da is spared in the process. (iii) When E(spl)m7 is expressed, it complexes with Sc or Da/Sc and promotes their degradation in a manner that requires the corepressor Groucho and the Sc SPTSS motif. Da/Sc reciprocally promotes E(spl)m7 degradation. Since E(spl)m7 is a direct target of Notch, the mutual destabilization of Sc and E(spl) may contribute in part to the highly conserved anti-neural activity of Notch. Sc variants lacking the SPTSS motif are dramatically stabilized and are hyperactive in transgenic flies. Our results propose a novel mechanism of regulation of neurogenesis, involving the stability of key players in the process.
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