ArticleDevelopment (Cambridge, England)2015
Dynamic visualization of transcription and RNA subcellular localization in zebrafish.
Article in Development (Cambridge, England), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 65 citations in OpenAlex.
- Real-time single-molecule imaging in zebrafish embryos uncovers noncanonical translation.Science advances · 2026Article
- Single-cell transcriptional dynamics in a living vertebrate.Cell systems · 2026Article
- Single-cell transcriptomes of zebrafish germline reveal progenitor types and feminization by Foxl2l.eLife · 2025Article
- A simple MiMIC-based approach for tagging endogenous genes to visualise live transcription in Drosophila.Development (Cambridge, England) · 2024Article
- Real-time single-molecule imaging of transcriptional regulatory networks in living cells.Nature reviews. Genetics · 2024Review
- Spatial organization and function of RNA molecules within phase-separated condensates in zebrafish are controlled by Dnd1.Developmental cell · 2023Article
- CRISPR-dCas13-tracing reveals transcriptional memory and limited mRNA export in developing zebrafish embryos.Genome biology · 2023Article
- In Situ Hybridization as a Method to Examine Gene Regulatory Activity In Vivo.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Article
- Dynamics ofProceedings of the National Academy of Sciences of the United States of America · 2022Article
- The Akt-mTOR Pathway Drives Myelin Sheath Growth by Regulating Cap-Dependent Translation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- RNA transport from transcription to localized translation: a single molecule perspective.RNA biology · 2021Review
- Mini-III RNase-based dual-color system forDevelopment (Cambridge, England) · 2020Article
- Capturing and Understanding the Dynamics and Heterogeneity of Gene Expression in the Living Cell.International journal of molecular sciences · 2020Review
- Bioorthogonal mRNA labeling at the poly(A) tail for imaging localization and dynamics in live zebrafish embryos.Chemical science · 2020Article
- Imaging nascent transcription in wholemount vertebrate embryos to characterize zygotic genome activation.Methods in enzymology · 2020Article
- Spatiotemporal Patterning of Zygotic Genome Activation in a Model Vertebrate Embryo.Developmental cell · 2019Article
- Mechanisms regulating zygotic genome activation.Nature reviews. Genetics · 2019Review
- Imaging cell-type-specific dynamics of mRNAs in living mouse brain.Methods (San Diego, Calif.) · 2019Article
- A cell cycle-coordinated Polymerase II transcription compartment encompasses gene expression before global genome activation.Nature communications · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Live imaging of transcription and RNA dynamics has been successful in cultured cells and tissues of vertebrates but is challenging to accomplish in vivo. The zebrafish offers important advantages to study these processes--optical transparency during embryogenesis, genetic tractability and rapid development. Therefore, to study transcription and RNA dynamics in an intact vertebrate organism, we have adapted the MS2 RNA-labeling system to zebrafish. By using this binary system to coexpress a fluorescent MS2 bacteriophage coat protein (MCP) and an RNA of interest tagged with multiple copies of the RNA hairpin MS2-binding site (MBS), live-cell imaging of RNA dynamics at single RNA molecule resolution has been achieved in other organisms. Here, using a Gateway-compatible MS2 labeling system, we generated stable transgenic zebrafish lines expressing MCP, validated the MBS-MCP interaction and applied the system to investigate zygotic genome activation (ZGA) and RNA localization in primordial germ cells (PGCs) in zebrafish. Although cleavage stage cells are initially transcriptionally silent, we detect transcription of MS2-tagged transcripts driven by the βactin promoter at ∼ 3-3.5 h post-fertilization, consistent with the previously reported ZGA. Furthermore, we show that MS2-tagged nanos3 3'UTR transcripts localize to PGCs, where they are diffusely cytoplasmic and within larger cytoplasmic accumulations reminiscent of those displayed by endogenous nanos3. These tools provide a new avenue for live-cell imaging of RNA molecules in an intact vertebrate. Together with new techniques for targeted genome editing, this system will be a valuable tool to tag and study the dynamics of endogenous RNAs during zebrafish developmental processes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.