ArticlePloS one2015
Testing pancreatic islet function at the single cell level by calcium influx with associated marker expression.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 39 citations in OpenAlex.
- Vascularization of human islets by adaptable endothelium for durable and functional subcutaneous engraftment.Science advances · 2025Article
- Immunotherapy With Low-Dose IL-2/CD25 Prevents β-Cell Dysfunction and Dysglycemia in Prediabetic NOD Mice.Diabetes · 2023Article
- TRPC3 Regulates Islet Beta-Cell Insulin Secretion.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Wnt4 is heterogeneously activated in maturing β-cells to control calcium signaling, metabolism and function.Nature communications · 2022Article
- Endoplasmic Reticulum (ER) Stress and Its Role in Pancreatic β-Cell Dysfunction and Senescence in Type 2 Diabetes.International journal of molecular sciences · 2022Review
- BACH2 inhibition reverses β cell failure in type 2 diabetes models.The Journal of clinical investigation · 2021Article
- Angiopoietins stimulate pancreatic islet development from stem cells.Scientific reports · 2021Article
- Signaling Molecules Regulating Pancreatic Endocrine Development from Pluripotent Stem Cell Differentiation.International journal of molecular sciences · 2020Review
- SIX2 Regulates Human β Cell Differentiation from Stem Cells and Functional Maturation In Vitro.Cell reports · 2020Article
- Glucose Response by Stem Cell-Derived β Cells In Vitro Is Inhibited by a Bottleneck in Glycolysis.Cell reports · 2020Article
- Intact pancreatic islets and dispersed beta-cells both generate intracellular calcium oscillations but differ in their responsiveness to glucose.Cell calcium · 2019Article
- Beta-cell excitability and excitability-driven diabetes in adult Zebrafish islets.Physiological reports · 2019Article
- Analysis of Beta-cell Function Using Single-cell Resolution Calcium Imaging in Zebrafish Islets.Journal of visualized experiments : JoVE · 2018Article
- The role of beta cell heterogeneity in islet function and insulin release.Journal of molecular endocrinology · 2018Review
- In vivo monitoring of intracellular CaIslets · 2018Article
- Pancreatic islet inflammation: an emerging role for chemokines.Journal of molecular endocrinology · 2017Review
- An optimized protocol for purification of functional islets of Langerhans.Laboratory investigation; a journal of technical methods and pathology · 2017Article
- Statistical approaches and software for clustering islet cell functional heterogeneity.Islets · 2016Article
- Single-cell approaches for molecular classification of endocrine tumors.Current opinion in oncology · 2016Review
- An Abbreviated Protocol for In Vitro Generation of Functional Human Embryonic Stem Cell-Derived Beta-Like Cells.PloS one · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Studying the response of islet cells to glucose stimulation is important for understanding cell function in healthy and disease states. Most functional assays are performed on whole islets or cell populations, resulting in averaged observations and loss of information at the single cell level. We demonstrate methods to examine calcium fluxing in individual cells of intact islets in response to multiple glucose challenges. Wild-type mouse islets predominantly contained cells that responded to three (out of three) sequential high glucose challenges, whereas cells of diabetic islets (db/db or NOD) responded less frequently or not at all. Imaged islets were also immunostained for endocrine markers to associate the calcium flux profile of individual cells with gene expression. Wild-type mouse islet cells that robustly fluxed calcium expressed β cell markers (INS/NKX6.1), whereas islet cells that inversely fluxed at low glucose expressed α cell markers (GCG). Diabetic mouse islets showed a higher proportion of dysfunctional β cells that responded poorly to glucose challenges. Most of the failed calcium influx responses in β cells were observed in the second and third high glucose challenges, emphasizing the importance of multiple sequential glucose challenges for assessing the full function of islet cells. Human islet cells were also assessed and showed functional α and β cells. This approach to analyze islet responses to multiple glucose challenges in correlation with gene expression assays expands the understanding of β cell function and the diseased state.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.