Evidence map›Paper›PMID 25900808›Full record

ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2015

Fabrication of 3-dimensional multicellular microvascular structures.

Sebastian F Barreto-Ortiz, Jamie Fradkin, Joon Eoh, Jacqueline Trivero, Matthew Davenport, Brian Ginn, Hai-Quan Mao, Sharon Gerecht

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
2.4field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 34 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Review
  5. Review
  6. Article
  7. Review
  8. Bioresorbable Polymeric Scaffold in Cardiovascular Applications.International journal of molecular sciences · 2020
    Review
  9. Article
  10. Regenerative and durable small-diameter graft as an arterial conduit.Proceedings of the National Academy of Sciences of the United States of America · 2019
    Article
  11. Tissue Engineering of the Microvasculature.Comprehensive Physiology · 2019
    Review
  12. Article
  13. Review
  14. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Sebastian F Barreto-Ortiz*Department of Chemical and Biomolecular Engineering, Johns Hopkins Physical Sciences-Oncology Center and Institute for NanoBioTechnology, and Departments of Biomedical Engineering and Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland, USA; and Translational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, Maryland, USA.
Jamie Fradkin*Department of Chemical and Biomolecular Engineering, Johns Hopkins Physical Sciences-Oncology Center and Institute for NanoBioTechnology, and Departments of Biomedical Engineering and Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland, USA; and Translational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, Maryland, USA.
Joon Eoh*Department of Chemical and Biomolecular Engineering, Johns Hopkins Physical Sciences-Oncology Center and Institute for NanoBioTechnology, and Departments of Biomedical Engineering and Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland, USA; and Translational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, Maryland, USA.
Jacqueline Trivero*Department of Chemical and Biomolecular Engineering, Johns Hopkins Physical Sciences-Oncology Center and Institute for NanoBioTechnology, and Departments of Biomedical Engineering and Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland, USA; and Translational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, Maryland, USA.
Matthew Davenport*Department of Chemical and Biomolecular Engineering, Johns Hopkins Physical Sciences-Oncology Center and Institute for NanoBioTechnology, and Departments of Biomedical Engineering and Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland, USA; and Translational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, Maryland, USA.
Brian Ginn*Department of Chemical and Biomolecular Engineering, Johns Hopkins Physical Sciences-Oncology Center and Institute for NanoBioTechnology, and Departments of Biomedical Engineering and Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland, USA; and Translational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, Maryland, USA.
Hai-Quan Mao*Department of Chemical and Biomolecular Engineering, Johns Hopkins Physical Sciences-Oncology Center and Institute for NanoBioTechnology, and Departments of Biomedical Engineering and Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland, USA; and Translational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, Maryland, USA.
Sharon Gerecht*Department of Chemical and Biomolecular Engineering, Johns Hopkins Physical Sciences-Oncology Center and Institute for NanoBioTechnology, and Departments of Biomedical Engineering and Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland, USA; and Translational Tissue Engineering Center, Johns Hopkins School of Medicine, Baltimore, Maryland, USA gerecht@jhu.edu.
Johns Hopkins University · US

Funding

TRANS NETWORK PROJECTSU54CA143868 · NCI · JOHNS HOPKINS UNIVERSITY · PI WIRTZ, DENIS · 2009 to 2013
$13.7M
Engineered Stem Cell Microenvironments for controlled VasculogenesisR01HL107938 · NHLBI · JOHNS HOPKINS UNIVERSITY · PI GERECHT, SHARON · 2011 to 2015
$2.1M
NCI NIH HHS U54 CA143868NCI NIH HHS U54CA143868NHLBI NIH HHS R01 HL107938NHLBI NIH HHS R01HL107938
6 · The paper itself

Abstract

Despite current advances in engineering blood vessels over 1 mm in diameter and the existing wealth of knowledge regarding capillary bed formation, studies for the development of microvasculature, the connecting bridge between them, have been extremely limited so far. Here, we evaluate the use of 3-dimensional (3D) microfibers fabricated by hydrogel electrospinning as templates for microvascular structure formation. We hypothesize that 3D microfibers improve extracellular matrix (ECM) deposition from vascular cells, enabling the formation of freestanding luminal multicellular microvasculature. Compared to 2-dimensional cultures, we demonstrate with confocal microscopy and RT-PCR that fibrin microfibers induce an increased ECM protein deposition by vascular cells, specifically endothelial colony-forming cells, pericytes, and vascular smooth muscle cells. These ECM proteins comprise different layers of the vascular wall including collagen types I, III, and IV, as well as elastin, fibronectin, and laminin. We further demonstrate the achievement of multicellular microvascular structures with an organized endothelium and a robust multicellular perivascular tunica media. This, along with the increased ECM deposition, allowed for the creation of self-supporting multilayered microvasculature with a distinct circular lumen following fibrin microfiber core removal. This approach presents an advancement toward the development of human microvasculature for basic and translational studies.

Indexed as

Cells, CulturedCollagenElastinEndothelial CellsEndothelium, VascularFibrinFibronectinsHumansLamininMicrofibrilsMicrovesselsMuscle, Smooth, VascularMyocytes, Smooth MuscleCollagenElastinFibrinFibronectinsLamininendothelial cellextracellular matrixfibrinmicrofiberperivascular cell

Identifiers

PMID25900808
PMCPMC4511194
OpenAlexW2075912084

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.