ArticleDiabetologia2015
Human islet function following 20 years of cryogenic biobanking.
Article in Diabetologia, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 43 citations in OpenAlex.
- Systematic review of islet cryopreservation.Islets · 2018Pooled it
- Trial
- From pancreas and islet resources to diabetes insights.Diabetologia · 2026Review
- The effect of hydroxyethyl starch as a cryopreservation agent during freezing of mouse pancreatic islets.Biochemistry and biophysics reports · 2024Article
- HumanIslets: An integrated platform for human islet data access and analysis.bioRxiv : the preprint server for biology · 2024Article
- Quantification of Secretory Granule Exocytosis by TIRF Imaging and Capacitance Measurements.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Article
- Every islet matters: improving the impact of human islet research.Nature metabolism · 2022Review
- Manufacturing clinical-grade human induced pluripotent stem cell-derived beta cells for diabetes treatment.Cell proliferation · 2022Review
- Article
- Genetic variant effects on gene expression in human pancreatic islets and their implications for T2D.Nature communications · 2020Article
- Patch-Seq Links Single-Cell Transcriptomes to Human Islet Dysfunction in Diabetes.Cell metabolism · 2020Article
- Paracrine control of α-cell glucagon exocytosis is compromised in human type-2 diabetes.Nature communications · 2020Article
- The challenge of correctly reporting hormones content and secretion in isolated human islets.Molecular metabolism · 2019Review
- The roles of reactive oxygen species and antioxidants in cryopreservation.Bioscience reports · 2019Review
- Article
- Cryopreserved Alginate-Encapsulated Islets Can Restore Euglycemia in a Diabetic Animal Model Better than Cryopreserved Non-encapsulated Islets.Cell medicine · 2019Article
- Type 2 diabetes risk alleles in PAM impact insulin release from human pancreatic β-cells.Nature genetics · 2018Article
- β-cell replacement sources for type 1 diabetes: a focus on pancreatic ductal cells.Therapeutic advances in endocrinology and metabolism · 2016Review
- Cell therapies for pancreatic beta-cell replenishment.Italian journal of pediatrics · 2016Review
Corrections and comments
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Authors and funding
17 authors at 7 institutions in 3 countries.
Funding
Abstract
aims/hypothesisThere are potential advantages to the low-temperature (-196 °C) banking of isolated islets, including the maintenance of viable islets for future research. We therefore assessed the in vitro and in vivo function of islets cryopreserved for nearly 20 years.
methodsHuman islets were cryopreserved from 1991 to 2001 and thawed between 2012 and 2014. These were characterised by immunostaining, patch-clamp electrophysiology, insulin secretion, transcriptome analysis and transplantation into a streptozotocin (STZ)-induced mouse model of diabetes.
resultsThe cryopreservation time was 17.6 ± 0.4 years (n = 43). The thawed islets stained positive with dithizone, contained insulin-positive and glucagon-positive cells, and displayed levels of apoptosis and transcriptome profiles similar to those of freshly isolated islets, although their insulin content was lower. The cryopreserved beta cells possessed ion channels and exocytotic responses identical to those of freshly isolated beta cells. Cells from a subset of five donors demonstrated similar perifusion insulin secretion profiles pre- and post-cryopreservation. The transplantation of cryopreserved islets into the diabetic mice improved their glucose tolerance but did not completely normalise their blood glucose levels. Circulating human insulin and insulin-positive grafts were detectable at 10 weeks post-transplantation. CONCLUSIONS/
interpretationWe have demonstrated the potential for long-term banking of human islets for research, which could enable the use of tissue from a large number of donors with future technologies to gain new insight into diabetes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.