ArticleRNA (New York, N.Y.)2015
The Ll.LtrB intron from Lactococcus lactis excises as circles in vivo: insights into the group II intron circularization pathway.
Article in RNA (New York, N.Y.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 21 citations in OpenAlex.
- Circular RNAs in coronary heart disease: From molecular mechanism to promising clinical application (Review).International journal of molecular medicine · 2025Review
- A linear and circular dual-conformation noncoding RNA involved in oxidative stress tolerance in Bacillus altitudinis.Nature communications · 2023Article
- Involvement of circRNAs in the Development of Heart Failure.International journal of molecular sciences · 2022Review
- Article
- Molecular characterization of both transesterification reactions of the group II intron circularization pathway.Nucleic acids research · 2021Article
- Review
- Group II Introns Generate Functional Chimeric Relaxase Enzymes with Modified Specificities through Exon Shuffling at Both the RNA and DNA Level.Molecular biology and evolution · 2021Article
- Diagnostic and prognostic value of circular RNAs in hepatocellular carcinoma.Journal of cellular and molecular medicine · 2020Review
- Article
- Article
- Bacterial group II introns generate genetic diversity by circularization and trans-splicing from a population of intron-invaded mRNAs.PLoS genetics · 2018Article
- Emerging function and potential diagnostic value of circular RNAs in cancer.Molecular cancer · 2018Review
- Deciphering the roles of lncRNAs in breast development and disease.Oncotarget · 2018Review
- Circular RNAs: Regulators of Cancer-Related Signaling Pathways and Potential Diagnostic Biomarkers for Human Cancers.Theranostics · 2017Review
- Recent horizontal transfer, functional adaptation and dissemination of a bacterial group II intron.BMC evolutionary biology · 2016Article
- Circularization pathway of a bacterial group II intron.Nucleic acids research · 2016Article
- Sequential splicing of a group II twintron in the marine cyanobacterium Trichodesmium.Scientific reports · 2015Article
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Authors and funding
4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Group II introns are large ribozymes that require the assistance of intron-encoded or free-standing maturases to splice from their pre-mRNAs in vivo. They mainly splice through the classical branching pathway, being released as RNA lariats. However, group II introns can also splice through secondary pathways like hydrolysis and circularization leading to the release of linear and circular introns, respectively. Here, we assessed in vivo splicing of various constructs of the Ll.LtrB group II intron from the Gram-positive bacterium Lactococcus lactis. The study of excised intron junctions revealed, in addition to branched intron lariats, the presence of perfect end-to-end intron circles and alternatively circularized introns. Removal of the branch point A residue prevented Ll.LtrB excision through the branching pathway but did not hinder intron circle formation. Complete intron RNA circles were found associated with the intron-encoded protein LtrA forming nevertheless inactive RNPs. Traces of double-stranded head-to-tail intron DNA junctions were also detected in L. lactis RNA and nucleic acid extracts. Some intron circles and alternatively circularized introns harbored variable number of non-encoded nucleotides at their splice junction. The presence of mRNA fragments at the splice junction of some intron RNA circles provides insights into the group II intron circularization pathway in bacteria.
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