ArticleMethods in molecular biology (Clifton, N.J.)2015
Detection of RNA-Protein Interactions Using Tethered RNA Affinity Capture.
Article in Methods in molecular biology (Clifton, N.J.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
5 citing papers in PubMed.
- Mycobacterium tuberculosis modulates NUDT21-mediated alternative polyadenylation to enhance FTH1 expression in macrophages and promotes intracellular growth.Frontiers in cellular and infection microbiology · 2025Article
- Rational Design of Aptamer-Tagged tRNAs.International journal of molecular sciences · 2020Article
- Gene Cascade Finder: A tool for identification of gene cascades and its application in Caenorhabditis elegans.PloS one · 2019Article
- Endocytosis and membrane receptor internalization: implication of F-BAR protein Carom.Frontiers in bioscience (Landmark edition) · 2017Review
- Analysis for Carom complex, signaling and function by database mining.Frontiers in bioscience (Landmark edition) · 2016Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Recent progress in large-scale nucleic acid analysis technology has revealed the presence of vast numbers of RNA species in cells, and extensive processing. To investigate the functions of these transcripts highly efficient methods are needed to analyze their interactions with RNA-binding proteins (RNBPs), and to understand the binding mechanisms. Many methods have been described to identify RNBPs, but none are wholly satisfactory, in part because RNAs are flexible macromolecules that adopt multiple conformations only some of which might bind to specific proteins. Here we describe a novel in vitro RNA-pull-down assay using tRNA scaffolded Streptavidin Aptamer (tRSA), to identify transcript specific RNA binding protein from mammalian cell lysates. The tRNA scaffold functions to stabilize the structure of the aptamer and the attached RNA, increasing the efficiency of the affinity purification.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.