Evidence map›Paper›PMID 26109048›Full record

ArticleGenes & development2015

Gata6 potently initiates reprograming of pluripotent and differentiated cells to extraembryonic endoderm stem cells.

Sissy E Wamaitha, Ignacio del Valle, Lily T Y Cho, Yingying Wei, Norah M E Fogarty, Paul Blakeley, Richard I Sherwood, Hongkai Ji, Kathy K Niakan

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Genes & development, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers.

0numbers the graph read from it
0cells of the map it votes in
84citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

84 citing papers in PubMed, 147 citations in OpenAlex.

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24 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Sissy E WamaithaMill Hill Laboratory, The Francis Crick Institute, London NW7 1AA, United Kingdom;
Ignacio del ValleMill Hill Laboratory, The Francis Crick Institute, London NW7 1AA, United Kingdom;
Lily T Y ChoMill Hill Laboratory, The Francis Crick Institute, London NW7 1AA, United Kingdom;
Yingying WeiDepartment of Biostatistics, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA;
Norah M E FogartyMill Hill Laboratory, The Francis Crick Institute, London NW7 1AA, United Kingdom;
Paul BlakeleyMill Hill Laboratory, The Francis Crick Institute, London NW7 1AA, United Kingdom;
Richard I SherwoodBrigham and Women's Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115, USA.
Hongkai JiDepartment of Biostatistics, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA;
Kathy K NiakanMill Hill Laboratory, The Francis Crick Institute, London NW7 1AA, United Kingdom;
The Francis Crick Institute · GBJohns Hopkins University · USHarvard University · US

Funding

Statistical and Computational Tools for Next-generation ChIP-seq ApplicationsR01HG006841 · NHGRI · JOHNS HOPKINS UNIVERSITY · PI JI, HONGKAI · 2012 to 2014
$951k
Cancer Research UKMedical Research Council MC_UP_1202/9NHGRI NIH HHS R01 HG006841NHGRI NIH HHS R01HG006841Wellcome Trust
6 · The paper itself

Abstract

Transcription factor-mediated reprograming is a powerful method to study cell fate changes. In this study, we demonstrate that the transcription factor Gata6 can initiate reprograming of multiple cell types to induced extraembryonic endoderm stem (iXEN) cells. Intriguingly, Gata6 is sufficient to drive iXEN cells from mouse pluripotent cells and differentiated neural cells. Furthermore, GATA6 induction in human embryonic stem (hES) cells also down-regulates pluripotency gene expression and up-regulates extraembryonic endoderm (ExEn) genes, revealing a conserved function in mediating this cell fate switch. Profiling transcriptional changes following Gata6 induction in mES cells reveals step-wise pluripotency factor disengagement, with initial repression of Nanog and Esrrb, then Sox2, and finally Oct4, alongside step-wise activation of ExEn genes. Chromatin immunoprecipitation and subsequent high-throughput sequencing analysis shows Gata6 enrichment near pluripotency and endoderm genes, suggesting that Gata6 functions as both a direct repressor and activator. Together, this demonstrates that Gata6 is a versatile and potent reprograming factor that can act alone to drive a cell fate switch from diverse cell types.

Indexed as

AnimalsBinding SitesCell DifferentiationCellular ReprogrammingEmbryonic Stem CellsEndodermFibroblast Growth Factor 4GATA4 Transcription FactorGATA6 Transcription FactorGene Expression Regulation, DevelopmentalGene Regulatory NetworksHumansMiceOctamer Transcription Factor-3Pluripotent Stem CellsProtein BindingFgf4 protein, mouseFibroblast Growth Factor 4Gata4 protein, mouseGATA4 Transcription FactorGata6 protein, mouseGATA6 Transcription FactorOctamer Transcription Factor-3Pou5f1 protein, mouseextraembryonic endodermGata6human embryonic stem cellsmouse embryonic stem cellspluripotencyreprograming

Identifiers

PMID26109048
PMCPMC4495396
OpenAlexW2112816839

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.