ArticleIntegrative biology : quantitative biosciences from nano to macro2015
Printing cancer cells into intact microvascular networks: a model for investigating cancer cell dynamics during angiogenesis.
Article in Integrative biology : quantitative biosciences from nano to macro, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 60 citations in OpenAlex.
- Controlled tumor heterogeneity in a co-culture system by 3D bio-printed tumor-on-chip model.Scientific reports · 2023Article
- Bioprinted Membranes for Corneal Tissue Engineering: A Review.Pharmaceutics · 2022Review
- A 3D Bioprinted in vitro Model of Neuroblastoma Recapitulates Dynamic Tumor-Endothelial Cell Interactions Contributing to Solid Tumor Aggressive Behavior.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- A Novel ex vivo Method for Investigating Vascularization of Transplanted Islets.Journal of vascular research · 2022Article
- Research Dynamics of Tissue Spheroids as Building Blocks: A Scientometric Analysis.International journal of bioprinting · 2022Article
- The Emerging Role of Decellularized Plant-Based Scaffolds as a New Biomaterial.International journal of molecular sciences · 2021Review
- Bioprinting on Live Tissue for Investigating Cancer Cell Dynamics.Tissue engineering. Part A · 2021Article
- Digitally Fabricated and Naturally Augmented In Vitro Tissues.Advanced healthcare materials · 2021Review
- Direct Bioprinting of 3D Multicellular Breast Spheroids onto Endothelial Networks.Journal of visualized experiments : JoVE · 2020Article
- Retrospective Comparison of Contrast-Enhanced Spectral Mammography with Digital Mammography in Assessing Tumor Size in 668 Cases of Breast Cancer.Medical science monitor : international medical journal of experimental and clinical research · 2020Article
- Prospects and Challenges of Translational Corneal Bioprinting.Bioengineering (Basel, Switzerland) · 2020Review
- Bioprinting Cell- and Spheroid-Laden Protein-Engineered Hydrogels as Tissue-on-Chip Platforms.Frontiers in bioengineering and biotechnology · 2020Article
- Thermal Bioprinting Causes Ample Alterations of Expression of LUCAT1, IL6, CCL26, and NRN1L Genes and Massive Phosphorylation of Critical Oncogenic Drug Resistance Pathways in Breast Cancer Cells.Frontiers in bioengineering and biotechnology · 2020Article
- Bioprinting of 3D breast epithelial spheroids for human cancer models.Biofabrication · 2019Article
- Understanding angiogenesis during aging: opportunities for discoveries and new models.Journal of applied physiology (Bethesda, Md. : 1985) · 2018Review
- Microstencil-based spatial immobilization of individual cells for single cell analysis.Biomicrofluidics · 2018Article
- Magnetic Resonance Imaging for tracking cellular patterns obtained by Laser-Assisted Bioprinting.Scientific reports · 2018Article
- Engineering Breast Cancer Microenvironments and 3D Bioprinting.Frontiers in bioengineering and biotechnology · 2018Review
- Methods of Micropatterning and Manipulation of Cells for Biomedical Applications.Micromachines · 2017Review
- Vascular Endothelial-Breast Epithelial Cell Coculture Model Created from 3D Cell Structures.ACS biomaterials science & engineering · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
While cancer cell invasion and metastasis are dependent on cancer cell-stroma, cancer cell-blood vessel, and cancer cell-lymphatic vessel interactions, our understanding of these interactions remain largely unknown. A need exists for physiologically-relevant models that more closely mimic the complexity of cancer cell dynamics in a real tissue environment. The objective of this study was to combine laser-based cell printing and tissue culture methods to create a novel ex vivo model in which cancer cell dynamics can be tracked during angiogenesis in an intact microvascular network. Laser direct-write (LDW) was utilized to reproducibly deposit breast cancer cells (MDA-MB-231 and MCF-7) and fibroblasts into spatially-defined patterns on cultured rat mesenteric tissues. In addition, heterogeneous patterns containing co-printed MDA-MB-231/fibroblasts or MDA-MB-231/MCF-7 cells were generated for fibroblast-directed and collective cell invasion models. Printed cells remained viable and the cells retained the ability to proliferate in serum-rich media conditions. Over a culture period of five days, time-lapse imaging confirmed fibroblast and MDA-MB-231 cell migration within the microvascular networks. Confocal microscopy indicated that printed MDA-MB-231 cells infiltrated the tissue thickness and were capable of interacting with endothelial cells. Angiogenic network growth in tissue areas containing printed cancer cells was characterized by significantly increased capillary sprouting compared to control tissue areas containing no printed cells. Our results establish an innovative ex vivo experimental platform that enables time-lapse evaluation of cancer cell dynamics during angiogenesis within a real microvascular network scenario.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.