Evidence map›Paper›PMID 26244913›Full record

ArticleJournal of biomedical optics2015

Probing differentiation in cancer cell lines by single-cell micro-Raman spectroscopy.

Surekha Barkur, Aseefhali Bankapur, Madhura Pradhan, Santhosh Chidangil, Deepak Mathur, Uma Ladiwala

Abstract read
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In one paragraph

Article in Journal of biomedical optics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Surekha BarkurManipal University, Department of Atomic and Molecular Physics, Manipal 576 104, India.
Aseefhali BankapurManipal University, Department of Atomic and Molecular Physics, Manipal 576 104, India.
Madhura PradhanUM-DAE Centre for Excellence in Basic Sciences, Kalina Campus, Mumbai 400 098, India.
Santhosh ChidangilManipal University, Department of Atomic and Molecular Physics, Manipal 576 104, India.
Deepak MathurTata Institute of Fundamental Research, 1 Homi Bhabha Road, Mumbai 400 005, India.
Uma LadiwalaUM-DAE Centre for Excellence in Basic Sciences, Kalina Campus, Mumbai 400 098, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Single-cell micro-Raman spectroscopy has been applied to explore cell differentiation in single, live, and malignant cells from two tumor cell lines. The spectra of differentiated cells exhibit substantial enhancement primarily in the intensities of protein peaks with concomitant decrease in intensities of O−P−O asymmetric stretching peaks in DNA/RNA. Principal component analyses show that the spectral score of differentiated cells tends to asymptotically approach that of spectra obtained from normal neural stem cells/progenitors. This lends credence to the notion that the observed spectral changes are specific to differentiation, since upon differentiation, malignant cells become less malignant and tend toward benignity.

Indexed as

AnimalsCell DifferentiationCell Line, TumorCell Transformation, NeoplasticNeoplasmsRatsRats, WistarReproducibility of ResultsSensitivity and SpecificitySpectrum Analysis, Raman

Identifiers

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.