ArticleBMC genomics2015
De novo sequencing and assembly analysis of transcriptome in the Sodom apple (Calotropis gigantea).
Article in BMC genomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
- From traditional uses to biochemical evidence: phytochemical diversity and bioactivities of Calotropis gigantea-a comprehensive update since 2013.Medicinal chemistry research : an international journal for rapid communications on design and mechanisms of action of biologically active agents · 2026Review
- Transcriptome Analysis of the Seed Shattering Mechanism inPlants (Basel, Switzerland) · 2024Article
- Characterisation of theBiodiversity data journal · 2024Article
- Transcript profiling of hairy vetch (Vicia villosa Roth) identified interesting genes for seed dormancy.The plant genome · 2023Article
- Integrated Analysis of microRNA and RNA-Seq Reveals Phenolic Acid Secretion Metabolism in Continuous Cropping ofPlants (Basel, Switzerland) · 2023Article
- Analysis of seed production and seed shattering in a new artificial grassland forage: pigeon pea.Frontiers in plant science · 2023Article
- Histological characteristics, cell wall hydrolytic enzyme activity, and transcriptome analysis with seed shattering ofFrontiers in plant science · 2022Article
- Article
- Genetic Diversity Analysis Reveals Genetic Differentiation and Strong Population Structure in Calotropis Plants.Scientific reports · 2018Article
- Article
- Transcriptome Analyses Reveal Candidate Pod Shattering-Associated Genes Involved in the Pod Ventral Sutures of Common Vetch (Frontiers in plant science · 2017Article
- De novo transcriptomic analysis of the female and male adults of the blood fluke Schistosoma turkestanicum.Parasites & vectors · 2016Article
- De Novo Sequencing and Assembly Analysis of the Pseudostellaria heterophylla Transcriptome.PloS one · 2016Article
- Transcriptome Characterization and Functional Marker Development in Sorghum Sudanense.PloS one · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe Sodom apple (Calotropis gigantea), a member of the Asclepiadaceae family, is a large evergreen shrub native to continental Asia and northern Africa. As an important medicinal shrub and a fiber resource plant, there is an urgent need for developing molecular markers to facilitate breeding and genetic improvement of varieties.
resultsIn this study, using the Illumina high throughput sequencing technique we obtained about 45 million paired end sequencing reads, De novo assembled and generated a total of 133,634 transcripts with a mean of 1837.47 bp in length. Based on protein homology searches against available databases, a total of 21,851 unigenes were functionally annotated. In particular, many transcripts that encode for putative proteins involved in fiber and secondary metabolite biosynthesis were identified and analyzed. Key fiber genes identified were validated experimentally through Real-Time PCR technique. Various transcription factors involved in regulating plant response to abiotic stress were also identified. In addition, based on the unigene sequences assembled, 11,623 microsatellites loci were detected, which provide very useful resources for developing microsatellite molecular markers.
conclusionThis study is the first report on transcriptome information in the Calotropis species and provides rich gene transcript resources for conducting further studies on understanding the molecular basis of fiber and secondary metabolite biosynthesis, serving the genetic improvement and resource utilization in Calotropis plants.
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Registered trials
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