Evidence map›Paper›PMID 26407694›Full record

ArticleThe Journal of general virology2015

Direct whole-genome deep-sequencing of human respiratory syncytial virus A and B from Vietnamese children identifies distinct patterns of inter- and intra-host evolution.

Lien Anh Ha Do, Andreas Wilm, H Rogier van Doorn, Ha Minh Lam, Shuzhen Sim, Rashmi Sukumaran, Anh Tuan Tran, Bach Hue Nguyen, Thi Thu Loan Tran, Quynh Huong Tran and 20 more

Abstract read
In one paragraph

Article in The Journal of general virology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

30 authors.

Lien Anh Ha DoOxford University Clinical Research Unit, Wellcome Trust Major Overseas Program, Ho Chi Minh City, Vietnam.
Andreas WilmGenome Institute of Singapore, Genome Building, 138672 Singapore.
H Rogier van DoornOxford University Clinical Research Unit, Wellcome Trust Major Overseas Program, Ho Chi Minh City, Vietnam.
Ha Minh LamOxford University Clinical Research Unit, Wellcome Trust Major Overseas Program, Ho Chi Minh City, Vietnam.
Shuzhen SimGenome Institute of Singapore, Genome Building, 138672 Singapore.
Rashmi SukumaranGenome Institute of Singapore, Genome Building, 138672 Singapore.
Anh Tuan TranChildren's Hospital 1, Ward 10, District 10, Ho Chi Minh City, Vietnam.
Bach Hue NguyenChildren's Hospital 1, Ward 10, District 10, Ho Chi Minh City, Vietnam.
Thi Thu Loan TranChildren's Hospital 2, Ben Nghe Ward, District 1, Ho Chi Minh City, Vietnam.
Quynh Huong TranChildren's Hospital 2, Ben Nghe Ward, District 1, Ho Chi Minh City, Vietnam.
Quoc Bao VoChildren's Hospital 2, Ben Nghe Ward, District 1, Ho Chi Minh City, Vietnam.
Nguyen Anh Tran DacChildren's Hospital 2, Ben Nghe Ward, District 1, Ho Chi Minh City, Vietnam.
Hong Nhien TrinhChildren's Hospital 1, Ward 10, District 10, Ho Chi Minh City, Vietnam.
Thi Thanh Hai NguyenChildren's Hospital 1, Ward 10, District 10, Ho Chi Minh City, Vietnam.
Bao Tinh Le BinhChildren's Hospital 1, Ward 10, District 10, Ho Chi Minh City, Vietnam.
Khanh LeChildren's Hospital 1, Ward 10, District 10, Ho Chi Minh City, Vietnam.
Minh Tien NguyenChildren's Hospital 1, Ward 10, District 10, Ho Chi Minh City, Vietnam.
Quang Tung ThaiChildren's Hospital 1, Ward 10, District 10, Ho Chi Minh City, Vietnam.
Thanh Vu VoChildren's Hospital 1, Ward 10, District 10, Ho Chi Minh City, Vietnam.
Ngoc Quang Minh NgoChildren's Hospital 1, Ward 10, District 10, Ho Chi Minh City, Vietnam.
Thi Kim Huyen DangChildren's Hospital 2, Ben Nghe Ward, District 1, Ho Chi Minh City, Vietnam.
Ngoc Huong CaoChildren's Hospital 2, Ben Nghe Ward, District 1, Ho Chi Minh City, Vietnam.
Thu Van TranChildren's Hospital 2, Ben Nghe Ward, District 1, Ho Chi Minh City, Vietnam.
Lu Viet HoChildren's Hospital 2, Ben Nghe Ward, District 1, Ho Chi Minh City, Vietnam.
Jeremy FarrarOxford University Clinical Research Unit, Wellcome Trust Major Overseas Program, Ho Chi Minh City, Vietnam.
Menno de JongOxford University Clinical Research Unit, Wellcome Trust Major Overseas Program, Ho Chi Minh City, Vietnam.
Swaine ChenGenome Institute of Singapore, Genome Building, 138672 Singapore.
Niranjan NagarajanGenome Institute of Singapore, Genome Building, 138672 Singapore.
Juliet E BryantOxford University Clinical Research Unit, Wellcome Trust Major Overseas Program, Ho Chi Minh City, Vietnam.
Martin L HibberdGenome Institute of Singapore, Genome Building, 138672 Singapore.

Funding

Wellcome Trust 089276
6 · The paper itself

Abstract

Human respiratory syncytial virus (RSV) is the major cause of lower respiratory tract infections in children ,2 years of age. Little is known about RSV intra-host genetic diversity over the course of infection or about the immune pressures that drive RSV molecular evolution. We performed whole-genome deep-sequencing on 53 RSV-positive samples (37 RSV subgroup A and 16 RSV subgroup B) collected from the upper airways of hospitalized children in southern Vietnam over two consecutive seasons. RSV A NA1 and RSV B BA9 were the predominant genotypes found in our samples, consistent with other reports on global RSV circulation during the same period. For both RSV A and B, the M gene was the most conserved, confirming its potential as a target for novel therapeutics. The G gene was the most variable and was the only gene under detectable positive selection. Further, positively selected sites inG were found in close proximity to and in some cases overlapped with predicted glycosylation motifs, suggesting that selection on amino acid glycosylation may drive viral genetic diversity. We further identified hotspots and coldspots of intra-host genetic diversity in the RSV genome, some of which may highlight previously unknown regions of functional importance.

Indexed as

Evolution, MolecularAmino Acid SequenceChildGene Expression Regulation, ViralGenetic VariationGenome, ViralGenotypeHumansModels, MolecularPhylogenyProtein ConformationRespiratory Syncytial Virus, HumanRespiratory Syncytial Virus InfectionsVietnamViral ProteinsViral Proteins

Identifiers

PMID26407694
PMCPMC4804761

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.