ArticleProceedings of the National Academy of Sciences of the United States of America2015
Discovery of progenitor cell signatures by time-series synexpression analysis during Drosophila embryonic cell immortalization.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Inter-organ steroid hormone signaling promotes myoblast fusion via direct transcriptional regulation of a single key effector gene.Current biology : CB · 2024Article
- The untapped potential of cell culture in disentangling insect-microbial relationships.Microbiome research reports · 2024Review
- A new continuous cell line from the pest insect, Anomala cuprea (Coleoptera; Scarabaeidae): emergence of contractile cells.In vitro cellular & developmental biology. Animal · 2022Article
- Establishment and a comparative transcriptomic analysis of a male-specific cell line from the African malaria mosquito Anopheles gambiae.Scientific reports · 2022Article
- A cis-regulatory element promoting increased transcription at low temperature in cultured ectothermic Drosophila cells.BMC genomics · 2021Article
- A time course transcriptomic analysis of host and injected oncogenic cells reveals new aspects ofProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Cell Lines for Honey Bee Virus Research.Viruses · 2020Review
- Generating and working with Drosophila cell cultures: Current challenges and opportunities.Wiley interdisciplinary reviews. Developmental biology · 2019Review
- Thawing, Culturing, and Cryopreserving Drosophila Cell Lines.Journal of visualized experiments : JoVE · 2019Article
- A molecular atlas of the developing ectoderm defines neural, neural crest, placode, and nonneural progenitor identity in vertebrates.PLoS biology · 2017Article
- Potential neoplastic evolution of Vero cells: in vivo and in vitro characterization.Cytotechnology · 2017Article
- The Drosophila Gene Expression Tool (DGET) for expression analyses.BMC bioinformatics · 2017Article
- Oncogenic transformation of Drosophila somatic cells induces a functional piRNA pathway.Genes & development · 2016Article
- Article
- Diverse Hormone Response Networks in 41 Independent Drosophila Cell Lines.G3 (Bethesda, Md.) · 2016Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors.
Funding
Abstract
The use of time series profiling to identify groups of functionally related genes (synexpression groups) is a powerful approach for the discovery of gene function. Here we apply this strategy during Ras(V12) immortalization of Drosophila embryonic cells, a phenomenon not well characterized. Using high-resolution transcriptional time-series datasets, we generated a gene network based on temporal expression profile similarities. This analysis revealed that common immortalized cells are related to adult muscle precursors (AMPs), a stem cell-like population contributing to adult muscles and sharing properties with vertebrate satellite cells. Remarkably, the immortalized cells retained the capacity for myogenic differentiation when treated with the steroid hormone ecdysone. Further, we validated in vivo the transcription factor CG9650, the ortholog of mammalian Bcl11a/b, as a regulator of AMP proliferation predicted by our analysis. Our study demonstrates the power of time series synexpression analysis to characterize Drosophila embryonic progenitor lines and identify stem/progenitor cell regulators.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.