Phosphorylation of bovine papillomavirus E1 by the protein kinase CK2 near the nuclear localization signal does not influence subcellular distribution of the protein in dividing cells.

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In one paragraph

Article in Archives of virology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact, top 96% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Michael R LentzDepartment of Biology, University of North Florida, 1 UNF Drive, Jacksonville, FL, 32224, USA. mlentz@unf.edu.ORCID http://orcid.org/0000-0002-8789-2309

Tess ShidelerDepartment of Biology, University of North Florida, 1 UNF Drive, Jacksonville, FL, 32224, USA.

University of North Florida · US

Funding

Regulation of Papillomavirus DNA ReplicationR15CA087051 · NCI · UNIVERSITY OF NORTH FLORIDA · PI LENTZ, MICHAEL R · 2002 to 2002

$134k

NCI NIH HHS R15 CA087051

6 · The paper itself

Abstract

The bovine papillomavirus E1 helicase is essential for viral replication. In dividing cells, DNA replication maintains, but does not increase, the viral genome copy number. Replication is limited by low E1 expression and an E1 nucleocytoplasmic shuttling mechanism. Shuttling is controlled in part by phosphorylation of E1 by cellular kinases. Here we investigate conserved sites for phosphorylation by kinase CK2 within the E1 nuclear localization signal. When these CK2 sites are mutated to either alanine or aspartic acid, no change in replication phenotype is observed, and there is no effect on the subcellular distribution of E1, which remains primarily nuclear. This demonstrates that phosphorylation of E1 by CK2 at these sites is not a factor in regulating viral DNA replication in dividing cells.

Indexed as

Amino Acid SequenceAnimalsBovine papillomavirus 1Casein Kinase IICattleCattle DiseasesCell NucleusDNA-Binding ProteinsMolecular Sequence DataNuclear Localization SignalsPapillomavirus InfectionsPhosphorylationProtein TransportViral ProteinsCasein Kinase IIDNA-Binding ProteinsE1 protein, Bovine papillomavirusNuclear Localization SignalsViral ProteinsDNA replicationE1 proteinKinase CK2PapillomavirusProtein phosphorylation

Identifiers

PMID26467928

DOI10.1007/s00705-015-2641-6

PMCPMC4833712

OpenAlexW1934392840

What Socratic holds

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LicenceTDM

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.