Evidence mapPaperPMID 26614261Full record

ArticleInternational urology and nephrology2016

The effects of ozone therapy on caspase pathways, TNF-α, and HIF-1α in diabetic nephropathy.

Aydın Güçlü, Haydar Ali Erken, Gülten Erken, Yavuz Dodurga, Arzu Yay, Özge Özçoban, Hasan Şimşek, Aydın Akçılar, Fatma Emel Koçak

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Article in International urology and nephrology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
1.7field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 49 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 1 country.

Aydın GüçlüDepartment of Nephrology, Faculty of Medicine, Ahi Evran University, Kırşehir, Turkey. aydinguclu@gmail.com.
Haydar Ali ErkenDepartment of Physiology, Faculty of Medicine, Balikesir University, Balikesir, Turkey.
Gülten ErkenDepartment of Physiology, Faculty of Medicine, Balikesir University, Balikesir, Turkey.
Yavuz DodurgaDepartment of Medical Biology, Pamukkale University School of Medicine, Denizli, Turkey.
Arzu YayDepartment of Histology and Embryology, Erciyes University School of Medicine, Kayseri, Turkey.
Özge ÖzçobanDepartment of Histology and Embryology, Erciyes University School of Medicine, Kayseri, Turkey.
Hasan ŞimşekDepartment of Physiology, Faculty of Medicine, Dumlupınar University, Kutahya, Turkey.
Aydın AkçılarExperimental Research Unit, Faculty of Medicine, Dumlupınar University, Kutahya, Turkey.
Fatma Emel KoçakDepartment of Biochemistry, Faculty of Medicine, Dumlupınar University, Kutahya, Turkey.
Dumlupinar University · TRBalıkesir University · TRErciyes University · TRAhi Evran University · TRPamukkale University · TR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAccelerated apoptosis plays a vital role in the development of diabetic vascular complications. Ozone may attenuate diabetic nephropathy by means of decreased apoptosis-related genes. The aim of our study was to investigate the effect of ozone therapy on streptozotocin-induced diabetic nephropathy in rats. Also the histopathological changes in diabetic kidney tissue with ozone treatment were evaluated.

methodsThe rats were randomly divided into six groups (n = 7): control (C), ozone (O), diabetic (D), ozone-treated diabetic (DO), insulin-treated diabetic (DI), and ozone- and insulin-treated diabetic (DOI). D, DI, and DOI groups were induced by a single intraperitoneal injection of streptozotocin. Ozone was given to the O, DO, and DOI groups. Group DI and DOI received subcutaneous (SC) insulin (3 IU). All animals received daily treatment for 6 weeks.

resultsExpressions of caspase-1-3-9, HIF-1α, and TNF-α genes were significantly higher in D group compared to C group (p < 0.05 for all). Ozone treatment resulted in significant decrease in the expressions of these genes in diabetic kidney tissue compared to both C and D group (p < 0.05 for all). Caspase-1-3-9, HIF-1α, and TNF-α gene expressions were found to be lower in DOI group compared to C group (p < 0.05 for all). Also adding ozone treatment to insulin therapy resulted in more significantly decrease in the expressions of these genes in diabetic tissue compared to only insulin-treated diabetic group (p < 0.05 for all). Regarding histological changes, ozone treatment resulted in decrease in the renal corpuscular inflammation and normal kidney morphology was observed. Both insulin and ozone therapies apparently improved kidney histological findings with less degenerated tubules and less inflammation of renal corpuscle compared to D, DO, and DI groups.

conclusionOzone therapy decreases the expressions of apoptotic genes in diabetic kidney tissue and improves the histopathological changes.

Indexed as

Diabetes Mellitus, ExperimentalGene Expression RegulationAnimalsApoptosisCaspasesDiabetic NephropathiesHypoxia-Inducible Factor 1, alpha SubunitIn Situ Nick-End LabelingMaleOxidants, PhotochemicalOzoneRatsRats, Sprague-DawleyReal-Time Polymerase Chain ReactionRNA, MessengerTumor Necrosis Factor-alphaCaspasesHif1a protein, ratHypoxia-Inducible Factor 1, alpha SubunitOxidants, PhotochemicalOzoneRNA, MessengerTumor Necrosis Factor-alphaCaspaseDiabetic nephropathyHIF-1αOzone therapyTNF-α

Identifiers

PMID26614261
OpenAlexW2271113257

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.