ArticleThe Plant journal : for cell and molecular biology2016
The PPR-SMR protein PPR53 enhances the stability and translation of specific chloroplast RNAs in maize.
Article in The Plant journal : for cell and molecular biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed.
- Designed Alleles of ZmRap2.7 Decouple the Trade-Off Between Early Flowering and Yield Penalty.Plant biotechnology journal · 2026Article
- Expanding the palette of cis-elements to tune the expression of chloroplast transgenes in tobacco.The Plant journal : for cell and molecular biology · 2026Article
- Pentatricopeptide repeat proteins in crops: Advances in functional mechanisms and breeding applications.Journal of integrative plant biology · 2026Review
- RNA-Binding Proteins in Dinoflagellates.International journal of molecular sciences · 2026Review
- Pentatricopeptide repeat proteins in plants: Cellular functions, action mechanisms, and potential applications.Plant communications · 2025Review
- Single-molecule visualization of sequence-specific RNA binding by a designer PPR protein.Nucleic acids research · 2024Article
- The chloroplast pentatricopeptide repeat protein RCN22 regulates tiller number in rice by affecting sugar levels via the TB1-RCN22-RbcL module.Plant communications · 2024Article
- Translational activation by a synthetic PPR protein elucidates control of psbA translation in Arabidopsis chloroplasts.The Plant cell · 2024Article
- Plastid retrograde signaling: A developmental perspective.The Plant cell · 2024Review
- Applications of Synthetic Pentatricopeptide Repeat Proteins.Plant & cell physiology · 2024Review
- Chloroplast C-to-U editing, regulated by a PPR protein BoYgl-2, is important for chlorophyll biosynthesis in cabbage.Horticulture research · 2024Article
- Chloroplast gene expression: Recent advances and perspectives.Plant communications · 2023Review
- Plant organellar RNA maturation.The Plant cell · 2023Review
- A translatome-transcriptome multi-omics gene regulatory network reveals the complicated functional landscape of maize.Genome biology · 2023Article
- Rearrangement with the nkd2 promoter contributed to allelic diversity of the r1 gene in maize (Zea mays).The Plant journal : for cell and molecular biology · 2022Article
- An Overview of Pentatricopeptide Repeat (PPR) Proteins in the MossPlants (Basel, Switzerland) · 2022Review
- Pentatricopeptide repeat protein CNS1 regulates maize mitochondrial complex III assembly and seed development.Plant physiology · 2022Article
- CAF Proteins Help SOT1 Regulate the Stability of ChloroplastInternational journal of molecular sciences · 2021Article
- Moss PPR-SMR protein PpPPR_64 influences the expression of a psaA-psaB-rps14 gene cluster and processing of the 23S-4.5S rRNA precursor in chloroplasts.Plant molecular biology · 2021Article
- Functioning of PPR Proteins in Organelle RNA Metabolism and Chloroplast Biogenesis.Frontiers in plant science · 2021Review
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4 authors.
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Abstract
Pentatricopeptide repeat (PPR) proteins are helical repeat proteins that bind RNA and influence gene expression in mitochondria and chloroplasts. Several PPR proteins in plants harbor a carboxy-terminal small-MutS-related (SMR) domain, but the functions of the SMR appendage are unknown. To address this issue, we studied a maize PPR-SMR protein denoted PPR53 (GRMZM2G438524), which is orthologous to the Arabidopsis protein SOT1 (AT5G46580). Null ppr53 alleles condition a chlorotic, seedling-lethal phenotype and a reduction in plastid ribosome content. Plastome-wide transcriptome and translatome analyses revealed strong defects in the expression of the ndhA and rrn23 genes, which were superimposed on secondary effects resulting from a decrease in plastid ribosome content. Transcripts with processed 5'-ends mapping approximately 70 nucleotides upstream of rrn23 and ndhA are absent in ppr53 mutants, and the translational efficiency of the residual ndhA mRNAs is reduced. Recombinant PPR53 binds with high affinity and specificity to the 5' proximal region of the PPR53-dependent 23S rRNA, suggesting that PPR53 protects this RNA via a barrier mechanism similar to that described for several PPR proteins lacking SMR motifs. However, recombinant PPR53 did not bind with high affinity to the ndhA 5' untranslated region, suggesting that PPR53's RNA-stabilization and translation-enhancing effects at the ndhA locus involve the participation of other factors.
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