Evidence map›Paper›PMID 26869834›Full record

ArticleJournal of ginseng research2015

A brief method for preparation of gintonin-enriched fraction from ginseng.

Sun-Hye Choi, Seok-Won Jung, Hyun-Sook Kim, Hyeon-Joong Kim, Byung-Hwan Lee, Joon Yong Kim, Jung-Hyun Kim, Sung Hee Hwang, Hyewon Rhim, Hyoung-Chun Kim and 1 more

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Article in Journal of ginseng research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed.

  1. Article
  2. Gintonin-EnrichedCurrent issues in molecular biology · 2024
    Article
  3. Article
  4. Article
  5. Article
  6. Visualization of the binding between gintonin, aJournal of ginseng research · 2022
    Article
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  12. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Sun-Hye ChoiGinsentology Research Laboratory and Department of Physiology, College of Veterinary Medicine and Bio/Molecular Informatics Center, Konkuk University, Seoul, South Korea.
Seok-Won JungGinsentology Research Laboratory and Department of Physiology, College of Veterinary Medicine and Bio/Molecular Informatics Center, Konkuk University, Seoul, South Korea.
Hyun-Sook KimGinsentology Research Laboratory and Department of Physiology, College of Veterinary Medicine and Bio/Molecular Informatics Center, Konkuk University, Seoul, South Korea.
Hyeon-Joong KimGinsentology Research Laboratory and Department of Physiology, College of Veterinary Medicine and Bio/Molecular Informatics Center, Konkuk University, Seoul, South Korea.
Byung-Hwan LeeGinsentology Research Laboratory and Department of Physiology, College of Veterinary Medicine and Bio/Molecular Informatics Center, Konkuk University, Seoul, South Korea.
Joon Yong KimVMTH, Konkuk University, Seoul, South Korea.
Jung-Hyun KimVMTH, Konkuk University, Seoul, South Korea.
Sung Hee HwangDepartment of Pharmaceutical Engineering, Sangji University, Wonju, South Korea.
Hyewon RhimLife Science Division, Korea Institute of Science and Technology, Seoul, South Korea.
Hyoung-Chun KimNeuropsychopharmacology and Toxicology Program, College of Pharmacy, Kangwon National University, Chuncheon, South Korea.
Seung-Yeol NahGinsentology Research Laboratory and Department of Physiology, College of Veterinary Medicine and Bio/Molecular Informatics Center, Konkuk University, Seoul, South Korea.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundGinseng has been used as a tonic for invigoration of the human body. In a previous report, we identified a novel candidate responsible for the tonic role of ginseng, designated gintonin. Gintonin induces [Ca(2+)]i transient in animal cells via lysophosphatidic acid receptor activation. Gintonin-mediated [Ca(2+)]i transient is linked to anti-Alzheimer's activity in transgenic Alzheimer's disease animal model. The previous method for gintonin preparation included multiple steps. The aim of this study is to develop a simple method of gintonin fraction with a high yield.

methodsWe developed a brief method to obtain gintonin using ethanol and water. We extracted ginseng with fermentation ethanol and fractionated the extract with water to obtain water-soluble and water-insoluble fractions. The water-insoluble precipitate, rather than the water-soluble supernatant, induced a large [Ca(2+)]i transient in primary astrocytes. We designated this fraction as gintonin-enriched fraction (GEF).

resultsThe yield of GEF was approximately 6-fold higher than that obtained in the previous gintonin preparation method. The apparent molecular weight of GEF, determined using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, was equivalent to that obtained in the previous gintonin preparation method. GEF induced [Ca(2+)]i transient in cortical astrocytes. The effective dose (ED50) was 0.3 ± 0.09 μg/mL. GEF used the same signal transduction pathway as gintonin during [Ca(2+)]i transient induction in mouse cortical astrocytes.

conclusionBecause GEF can be prepared through water precipitation of ginseng ethanol extract and is easily reproducible with high yield, it could be commercially utilized for the development of gintonin-derived functional health food and natural medicine.

Indexed as

ethanol extractionGEFginsengH2O precipitation

Identifiers

PMID26869834
PMCPMC4593782

What Socratic holds

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LicenceCC BY-NC-ND
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.