ArticleThe Biochemical journal1989
The role of protein kinase C in cholinergic stimulation of insulin secretion from rat islets of Langerhans.
Article in The Biochemical journal, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 71 citations in OpenAlex.
- The adhesion receptor GPR56 is activated by extracellular matrix collagen III to improve β-cell function.Cellular and molecular life sciences : CMLS · 2018Article
- Multivesicular exocytosis in rat pancreatic beta cells.Diabetologia · 2012Article
- Kisspeptin stimulation of insulin secretion: mechanisms of action in mouse islets and rats.Diabetologia · 2009Article
- Muscarinic agonists activate Ca2+ store-operated and -independent ionic currents in insulin-secreting HIT-T15 cells and mouse pancreatic beta-cells.The Journal of membrane biology · 2004Article
- Leptin constrains acetylcholine-induced insulin secretion from pancreatic islets of ob/ob mice.The Journal of clinical investigation · 1997Article
- Muscarinic activation of Ca2+/calmodulin-dependent protein kinase II in pancreatic islets. Temporal dissociation of kinase activation and insulin secretion.The Biochemical journal · 1996Article
- Two distinct modes of Ca2+ signalling by ACh in rat pancreatic beta-cells: concentration, glucose dependence and Ca2+ origin.The Journal of physiology · 1995Article
- Inhibition of glucose-stimulated insulin secretion by Ro 31-8220, a protein kinase C inhibitor.Endocrine · 1995Article
- Activation of protein kinase C partially alleviates noradrenaline inhibition of insulin secretion.The Biochemical journal · 1993Article
- The role of protein kinase C in insulin biosynthesis.Acta diabetologica · 1993Article
- Mechanisms of the stimulation of insulin release by oxytocin in normal mouse islets.The Biochemical journal · 1991Article
- Potentiation of stimulus-induced insulin secretion in protein kinase C-deficient RINm5F cells.The Biochemical journal · 1990Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The role of the Ca2+/phospholipid-dependent protein kinase C (PKC) in cholinergic potentiation of insulin release was investigated by measuring islet PKC activity and insulin secretion in response to carbachol (CCh), a cholinergic agonist. CCh caused a dose-dependent increase in insulin secretion from cultured rat islets at stimulatory glucose concentrations (greater than or equal to 7 mM), with maximal effects observed at 100 microM. Short-term exposure (5 min) of islets to 500 microM-CCh at 2 mM- or 20 mM-glucose resulted in redistribution of islet PKC activity from a predominantly cytosolic location to a membrane-associated form. Prolonged exposure (greater than 20 h) of islets to 200 nM-phorbol myristate acetate caused a virtual depletion of PKC activity associated with the islet cytosolic fraction. Under these conditions of PKC down-regulation, the potentiation of glucose-stimulated insulin secretion by CCh (500 microM) was significantly decreased, but not abolished. CCh stimulated the hydrolysis of inositol phospholipids in both normal and PKC-depleted islets, as assessed by the generation of radiolabelled inositol phosphates. These results suggest that the potentiation of glucose-induced insulin secretion by cholinergic agonists is partly mediated by activation of PKC as a consequence of phospholipid hydrolysis.
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